CD157在RPE细胞中的表达及对细胞迁移、黏附的影响  

CD157 expression and its effects on migration and adhesion of human retinal pigment epithelial cells

作　　者：李印[1,2] 李拓[1,2] 宋秀胜[1,2] 李家璋[1,2] 吴青松[1,2] 李红艳[1,2] 贺涛[3] 

机构地区：[1]恩施州中心医院眼科中心 [2]武汉大学恩施临床学院眼科中心,湖北省恩施市445000 [3]武汉大学人民医院眼科,湖北省武汉市430060

出　　处：《眼科新进展》2012年第11期1006-1009,共4页Recent Advances in Ophthalmology

基　　金：湖北省卫生厅科研项目(编号:JX1B110)~~

摘　　要：目的探讨视网膜色素上皮(retinal pigment epithelial,RPE)细胞表面是否有CD157的表达及其对RPE细胞黏附和迁移的作用。方法分别采用免疫组织化学、West-ern-blot、RT-PCR验证RPE细胞表面有无CD157的表达。取500×103个RPE细胞用10mg.L-1CD157抗体处理20min后种植于8μm孔径的Tanswell小室,置37℃、含体积分数5%CO2培养箱孵育18h检测细胞迁移变化;100×103个细胞用10mg.L-1CD157抗体处理20min后种植于10mg.L-1FN包被的Tanswell小室,37℃、含体积分数5%CO2培养箱孵育1h检测细胞黏附能力变化。迁移和黏附实验均用β-actin鼠抗作对照。结果免疫组织化学、Western-blot及RT-PCR均证实RPE细胞有CD157的表达。与对照组相比,CD157抗体刺激RPE细胞后,细胞的迁移(实验组迁移细胞数:203±36、219±42、225±37;对照组迁移细胞数:384±46、357±38、362±57)和黏附(实验组黏附细胞数:222±20、243±34、237±26;对照组黏附细胞数:330±56、353±44、347±38)能力均下降,差异均有统计学意义(均为P<0.05)。结论 RPE细胞表面可表达CD157,其与配体结合后对RPE细胞的迁移和黏附起抑制作用。Objective To investigated the expression of CD157 in retinal pigment epithelial（RPE） cells and its effects on RPE cells adhesion and migration. Methods Immunohistochemical, Western-blot and RT-PCR were utilized to detect the expression of CD157 in RPE cells. A total of 50 x 103 cells were pretreated for 20 minutes by 10 mg · L-1 CD157 antibody and then planted into 8 μm aperture Tanswell unit,incuba- ted in 37 ℃ 5% CO2 incubator for 18 hours,the RPE cells migration was observed. A to- tal of 100 × 103 cells were pretreated for 20 minutes by 10 mg · L- 1 CD157 antibody and then planted into FN-coated Tanswell unit,incubated 1 hour in 37 ℃ 5% CO2 incubator to detect adhesion ability. Anti-murine β-actin was set as control antibody in migration and adhesion experiments. Results Immunohistochemical, Western-blot and RT-PCR techniques confirmed the expression of CD157 on RPE cells. Compared with the control group,average migration（ experimental group cells：203± 36,219 ± 42,237 ± 26;Control group cells： 384 ± 46,353 ± 44,362 ± 57 ） and adhesion （ experimental group cells： 222 ± 20,243 ± 34,237 ± 26 ; Control group cells： 330± 56,353 ± 44,347 ± 38 ） abilities of the RPE cells pretreated with CD157 antibody decreased, there were statistical differences （ both P 〈 0.05 ）. Conelusion RPE cells express CD157, which can inhibit the migration and adhesion of RPE cells after binding to the ligand.

关 键 词：CD157 视网膜色素上皮细胞 迁移 黏附 

分 类 号：R692.31[医药卫生—泌尿科学]