风信子根尖预处理及核型分析  

Pretreatment Reagents for Hyacinthus Root Tip and Karyotype Analysis

作　　者：胡凤荣[1] 王斐[1] 鲍仁蕾[1] 谢蔚然[1] 

机构地区：[1]南京林业大学风景园林学院,南京210037

出　　处：《西北植物学报》2012年第10期2030-2034,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国博士后科学基金面上项目(20100470217);江苏省高校优势学科建设工程资助项目

摘　　要：采用秋水仙素、8-羟基喹啉、放线菌酮3种试剂及秋水仙素与8-羟基喹啉的混合液、冰水混合物等,按不同时间梯度对风信子‘Pink Pearl’根尖进行预处理,以获得进行染色体分析的清晰制片,为风信子品种染色体分析及其微结构研究奠定基础。结果表明:‘Pink Pearl’根尖经5种预处理后,再经固定、解离、染色、压片均可获得分散良好、形态正常、易于计数并进行核型分析的高质量染色体制片。秋水仙素和8-羟基喹啉的最适预处理时间分别是12h、20h;放线菌酮、秋水仙素与8-羟基喹啉混合液、冰水混合物的最适预处理时间均是24h。染色体核型分析表明:风信子的核型公式为2n=2x=16=6m+4sm+2smsat+4st;核不对称系数为60.2%,为2C核型。Hyacinth cultivar ‘Pink Pearl’ root tips were pretreated with five reagents including colchicine,8-hydroxyquinoline,cycloheximide,mixture of colchicine and 8-hydroxyquinoline and ice-water mixture by different time grads to obtain well dispersed chromosome.These results laid a foundation for the hyacinth chromosome analysis and fine structure researches.The results showed that after fixed with Carnoy fixative I,dissociated,dyed,squashed,the root tips pretreated with these 5 reagents all could be used in chromosomes preparation,which the chromosomes spreader well,given a normal form,and were easy for counting and karyotype analysis.The most favorable treated time for colchicine and 8-hydroxyquinoline was 12 h and 20 h,respectively;and for the other three reagents cycloheximide,mixture of colchicine and 8-hydroxyquinoline and ice-water mixture,the most favorable treated time was all 24 h.Hyacinth karyotype analysis was carried out with chromosome from root tip cells.The result showed that the karyotype formula was 2n=2x=16=6m＋4sm＋2smsat＋4st.The asymmetry index was 60.2% and belonged to category 2C of Stebbins.

关 键 词：风信子 根尖 预处理 核型分析 

分 类 号：Q343.22[生物学—遗传学]