黄条金刚竹对高浓度CO_2和O_3及其复合作用的生理响应  

Physiological Responses of Pleioblastus kongosanensis to the Elevated Atmospheric Ozone and CO_2 Alone and in Combination

作　　者：庄明浩[1,2] 陈双林[1] 李迎春[1] 郭子武[1] 杨清平[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]中国林业科学研究院亚热带林业实验中心,江西分宜336600

出　　处：《西北植物学报》2012年第10期2061-2067,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家公益性行业(林业)科研专项(201004008);中央级公益性科研院所基本科研业务费专项资金(RISF6915);浙江省中国林业科学研究院省院合作项目(2010SY01)

摘　　要：以黄条金刚竹为试材,环境背景大气为对照,应用开顶式气室(OTCs)熏蒸法模拟大气高浓度CO2(700μmol.mol-1)、O3(100nmol.mol-1)及其复合作用情景,分析叶片光合色素、膜脂过氧化及抗氧化酶等的变化规律。结果显示:(1)与对照相比,高浓度O3处理103d的黄条金刚竹叶片叶绿素a、叶绿素b、总叶绿素含量和叶绿素a/b、叶绿素/类胡萝卜素及SOD、POD、CAT活性均显著下降,而超氧阴离子和丙二醛含量、相对电导率、APX活性均显著升高,类胡萝卜素含量变化不明显。(2)与对照相比,同期高浓度CO2处理的叶片叶绿素a、叶绿素b、总叶绿素含量和叶绿素/类胡萝卜素均显著升高,而叶绿素a/b和超氧阴离子、丙二醛含量及SOD、POD、CAT、APX活性显著降低,相对电导率和类胡萝卜素含量变化不明显。(3)高浓度O3和CO2复合作用下,除叶绿素a/b和CAT活性显著下降外,其余测定指标均与对照无明显变化。研究表明:高浓度O3使黄条金刚竹叶片活性氧产生速率提高,抗氧化酶活性和光合色素含量降低,膜脂过氧化程度加剧,膜结构破坏,表现出严重的伤害效应;而高浓度CO2能降低叶片活性氧产生速率,减轻膜脂过氧化程度,提高光合色素含量,表现出保护效应;高浓度O3和CO2复合处理能使叶片维持比高浓度O3处理更高的光合色素含量和抗氧化酶活性,即高浓度CO2能在一定程度上有效地缓解高浓度O3对黄条金刚竹所造成的生理伤害。The photosynthetic pigment,lipid peroxidation and anti-oxidation enzymes in the leaf of Pleioblastus kongosanensis were analyzed under the stress of elevated concentration of CO2 （700 μmol·mol-1） and O3 （100 nmol·mol-1） in open-top chambers （OTCs）.After samples treated for 103 days and compared to the control,the results showed that：（1）In the elevated O3 treatment groups,the contents of Chl a,Chl b,total Chl and the ratio of Chl a/Chl b,Chl/Car,the activities of SOD,POD and CAT were decreased significantly,while the contents of O-·2,MDA,Car,relative electrical conductivity and activity of APX were increased significantly;（2）In the elevated CO2 treatment groups,the contents of Chl a,Chl b,total Chl and the ratio of Chl/Car were increased significantly,and the ratio of Chl a/Chl b,contents of O-·2 and MDA,activities of SOD,POD,CAT,APX were decreased significantly and relative electrical conductivity,content of Car had no significant difference;（3）In the elevated O2 and CO2 combined treatments,except Chl/Car ratio and CAT activity were decreased significantly,other physiological indicators had no significant differences.The results showed that the activities of anti-oxidation were significantly decreased,at the same time the O3 and malondialdehyde contents were increased significantly by elevated O3,so that the cell membrane structure was damaged seriously which represents injured effects to P.kongosanensis.Reactive oxygen species were decreased significantly by elevated CO2,which presents protective efficacy on P.kongosanensis.In combined stresses of elevated CO2 and O3,the leaves of P.kongosanensis could maintain high levels of photosynthetic pigment content and antioxidant activities to effectively regulate reactive oxygen species generation and clearing balance,indicating that elevated CO2 could ameliorate the oxidative stress in certain degree.

关 键 词：黄条金刚竹 O3浓度 CO2浓度 光合色素 膜脂过氧化 抗氧化酶 

分 类 号：Q945.79[生物学—植物学]