达乌尔黄鼠对溴敌隆敏感性的测定  

The determination on sensitive background of S. dauricus to Bromadiolone

作　　者：陈显赫[1] 孙启廷[1] 张雁冰[1] 段天一[1] 

机构地区：[1]中国疾病预防控制中心鼠疫布氏菌病预防控制基地,吉林白城137000

出　　处：《中国地方病防治》2012年第5期353-354,共2页Chinese Journal of Control of Endemic Diseases

摘　　要：目的确立达乌尔黄鼠对溴敌隆的敏感本底(即食毒期),为检测达乌尔黄鼠对溴敌隆抗药性提供科学的理论依据。方法通过摄食试验,以世界卫生组织(WHO)提供的方法,ED95置信上限确立最适宜的灭鼠剂量;通过食毒期与其对应的死亡率,用Litchfield方法计算出达乌尔黄鼠对第2代抗凝血灭鼠剂的敏感本底。结果达乌尔黄鼠对溴敌隆敏感本底测定最适宜的剂量为4.87 mg/kg,食毒期为20 d。测定中动物死亡高峰出现在饲毒后第3～4 d,第10 d全部死亡。结论以0.01%浓度配制毒饵进行敏感性测定,以20 d为食毒期作为标准进行达乌尔黄鼠对溴敌隆的抗药性调查具有实际意义。Objective To establish sensitive background of S.dauricus to Bromadiolone and to provide scientific theoretical basis for checking resistance of S.dauricus to Bromadiolone.Methods With alternative feeding test and methods of WHO,upper limit of 95% effective dose were established as the most suitable dose for eliminating mice;With the methods of Litchfield,the sensitive background of S.dauricus to the second generation anticoagulant poison were calculated by eating poison period with death rate.Results The most dose was 4.87mg/kg which sensitive background of Bromadiolone was determined on S.dauricus and eating poison period was twenty days.The rush hour of animal death arised in the third and fourth days and animals died overall in the seventh after feeding poisons.Conclusions Investigating resistance of S.dauricus to Bromadiolone has practical meaning with 0.01% baits for sensitive determination and eating poison period 20 days as standard.

关 键 词：达乌尔黄鼠 抗药性 溴敌隆 敏感本底 

分 类 号：R516.8[医药卫生—内科学]