利用高世代回交群体对大豆小粒性状的基因型分析及QTL定位  

Genotype analysis and QTL mapping small seed size soybean with advanced backcross population

作　　者：曾庆力[1] 蒋洪蔚[1,2] 刘春燕[2] 范冬梅[1] 马占洲[1] 王宏光[1] 胡国华[2] 陈庆山[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090

出　　处：《中国油料作物学报》2012年第5期473-477,共5页Chinese Journal of Oil Crop Sciences

基　　金：十二五国家科技支撑计划(2011BAD35B06);国家现代农业产业体系专项(CARS-04-02A);黑龙江省普通高等学校新世纪优秀人才培养计划(1252-NCET-004)

摘　　要：利用野生型大豆ZYD00006(供体亲本)与主栽品种绥农14(轮回亲本)所构建的高世代回交导入系,经过严格的百粒重筛选鉴定,得到43个百粒重性状明显小于轮回亲本的导入系个体。利用这套选择群体结合随机对照群体和基因型分析,通过基于遗传搭车原理的卡方分析,检测到分布于7个连锁群上的9个与大豆小粒性状相关的QTL位点,对小粒性状表现为正效应,为大豆小粒性状分子辅助育种提供有用的分子标记。A set of advanced backcross population were constructed with Suinong 14 as recurrent parent and wild soybean ZYD00006 as donor parent.After comparing with recurrent parent Suinong 14,43 BC3F2 individuals with small seed size were screened out,and another 43 individuals from BC3F2 were used as control（random population）.Nine QTLs（quantitative trait loci） on seven linkage groups controlling small seed size were detected with genotype analysis by chi-square on genetic hitchhiking,and these QTLs showed positive effect on small seed size.Advanced backcrossing lines were used for mapping QTL related to small seed size,and could benefit molecular assisted breeding.

关 键 词：大豆 导入系 小粒 QTL定位 

分 类 号：S565.103[农业科学—作物学]