反相高效液相色谱法测定长春西汀注射液的含量  

Determination of Vinpocetine in Vinpocetine Injection by RP-HPLC

作　　者：吴朝晖[1] 李根[2] 周静[3] 

机构地区：[1]福建医科大学附属第一医院,福建福州350004 [2]四川省成都市妇幼保健院,四川成都610041 [3]四川大学华西药学院,四川成都610041

出　　处：《中国药业》2012年第21期19-20,共2页China Pharmaceuticals

摘　　要：目的建立测定长春西汀注射液中长春西汀含量的反相高效液相色谱法。方法色谱柱为Agilent C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-13 mmol/L碳酸铵水溶液-四氢呋喃(100∶16∶5),流速为1.0 mL/min,检测波长为274 nm。结果长春西汀检测质量浓度在0.2～1.0 g/L范围内与峰面积呈良好线性关系(r=0.999 8);平均回收率为99.35%,RSD=0.76%(n=9)。结论该方法简便、准确、重现性好,可用于该制剂的质量控制。Objective To establish an RP-HPLC method for determining the content of vinpocetine in Vinpocetine Injection. Memods RP-HPLC was performed on the Agilent C18 column（250 mm×4. 6 mm, 5 μm）. The mobile phase was methanol-13 mmol/L ammonium carbonate solution-tetrahydrofuran（100 ： 16 ： 5）. The flow rate was 1.0 mL/min. The detective wavelength was set at 274 nm. Results The mass concentrations of vinpoeetine within the range of 0. 2-1.0 g/L showed good linearity with the integral value of the peak area（ r =0. 999 8）. The average recovery rate was 99.35%, RSD was 0.76% （n =9）. Conclusion This method is simple, accurate and well reproducible, and can be used for the quality control of Vinpocetine Injection.

关 键 词：长春西汀注射液 长春西汀 反相高效液相色谱法 含量测定 

分 类 号：R969.4[医药卫生—药理学] R972.4[医药卫生—药学]