腺病毒介导hVEGF编码基因转染骨髓基质干细胞的实验研究  

Experimental study on recombinant adenovirus-mediated hVEGF coding gene in bone marrow stromal cells

作　　者：何玺君 李慧勇 彭余江 朱良才 王永青 段虹宇 陈慧慧 杨帆 

机构地区：[1]浙江省温岭市第一人民医院,温岭317500

出　　处：《浙江创伤外科》2012年第5期596-598,共3页Zhejiang Journal of Traumatic Surgery

摘　　要：目的应用携带hVEGF基因的重组腺病毒载体转染大鼠骨髓基质干细胞(BMSCs),观察hVEGF的表达情况。方法体外培养大鼠骨髓基质干细胞,含hVEGF基因的重组腺病毒转染骨髓基质干细胞,转染后在荧光显微镜下观察并采用ELISA法检测hVEGF的表达水平。结果 rAd-hVEGF转染骨髓基质干细胞后,ELISA检测发现转染组上清液中hVEGF蛋白分泌量明显高于对照组(P<0.01)。结论 rAd-hVEGF转染大鼠骨髓基质干细胞能够表达并分泌hVEGF,为缺血性脑血管疾病的基因治疗提供可行性研究。Objective To transfect rAd-hVEGF into the bone marrow stromal cells （BMSCs） of SD rats, and then to assay the expression of VEGF. Methods rAd-hVEGF transfected BMSCs were cultured in vitro. The expression of VEGF in BMSCs after transfection was determined by observing the expression of GFP. ELISA method was applied to assay the secretion of hVEGF. Results EL1SA analysis showed the quantity of expression of hVEGF in transfection group was higher than that in control group （P〈0.01）. Conclusion Exogenous hVEGF can be expressed in BMSCs, which provided the basis of ischemic cerebrovaseular disease＇s gene therapy.

关 键 词：hVEGF基因 重组腺病毒 转染 骨髓基质干细胞 基因治疗 缺血性脑血管疾病 

分 类 号：R68[医药卫生—骨科学]