谷氨酰胺对腹部开放伤合并海水浸泡犬肠功能的影响研究  

Effects of glutamine on intestinal functions of dogs with abdominal open injury coupled with seawater immersion

作　　者：李欣[1] 吴姗珊[1] 刘超群[1] 浦江[1] 付山峰[1] 段蕴铀[1] 孙涛[1] 

机构地区：[1]海军总医院消化内科,北京100048

出　　处：《中华航海医学与高气压医学杂志》2012年第5期271-275,283,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：海后科研课题（04-3304）

摘　　要：目的探讨谷氨酰胺对腹部开放伤后海水浸泡实验犬肠功能的影响。方法30只成年犬致腹部开放伤，按数字表法随机分为海水浸泡组（对照组）、普通治疗组（常规组）和谷氨酰胺治疗组（观察组），每组10只。于致伤前及致伤后定时测定犬的血压、心率、呼吸、腹围、肠鸣音，取血测定内毒素、D-乳酸、双胺氧化酶（DAO）、肿瘤坏死因子．（TNF-a）、白细胞介素．1（IL-1）和尿乳果糖／甘露醇（L／M），24h后取小肠组织进行病理检查并测量肠黏膜厚度和绒毛高度。结果与常规组比较，观察组平均动脉压、心率、呼吸、肠鸣音和腹围差异均无统计学意义（P〉0．05），对照组与常规组、观察组比较，平均动脉压、肠鸣音差异均有统计学意义（P〈0．05），致伤后12h和24h时间点心率差异均有统计学意义（P〈0．05），致伤后24h时间点呼吸差异有统计学意义（P〈0．05），致伤后24h时间点腹围差异有统计学意义（P〈0．05）。内毒素：对照组与常规组、观察组比较，致伤后12h和24h时间点差异均有统计学意义（P〈0．05），观察组与对照组、常规组比较，在24h时间点差异均有统计学意义（P〈0．05）。D-乳酸：致伤后12h时间点，常规组与对照组、观察组差异均有统计学意义（P〈0．05），观察组和对照组之间差异无统计学意义（P〉0．05）；致伤后24h时间点，常规组与对照组、观察组差异均有统计学意义（P〈0．05），观察组和对照组之间差异无统计学意义（P〉0．05）。DAO：观察组与对照组、常规组比较，致伤后8h与24h时间点差异均有统计学意义（P〈0．05）。TNF-a：观察组与对照组、常规组比较，致伤后12h与24h时间点差异均有统计学意义（P〈0．05）。IL-1：致伤后12h时间点，常规组[（8．71±3．13）ng／L]与对照组[（7．40±2．71）ng／L]、观察组[（6．34±1．Objective To investigate the effects of glutamine on intestinal functions of dogs following abdominal open injury coupled with seawater immersion. Methods Following open abdominal injury, 30 adult dogs were randomly divided into 3 groups： the seawater immersion group （the control group ）, the routine treatment group （the conventional group ） and the glutamine treatment group （the observation group ）, each consisting of 10 animals. Blood pressure, heart rate, respiration, abdominal circumference, bowel sound were monitored both before and after injury. Blood samples were collected for the detection of endotoxin, D-lactate, diamine oxidase （DAO） , tumor necrosis factor alpha （TNF-a）, interleukin-1 （IL-1） and lactulose/mannitol（L/M） ratio. After 24 hours, small intestine tissues were taken for pathological detection and measurement of intestinal mucosal thickness and villous height. Results When compared with the conventional group, no statistically significant differences could be seen in mean artery pressure （MAP）, heart rate, respiration, bowel sound and abdominal circumference for the observation group （P 〉 0. 05 ）. When compared with the conventional group and the observation group, significant differences could be noted in MAP and bowel sound for the control group（P 〈 0.05 ）. And stalistical significance could also be noted for heart rate, respiration and abdominal circumference at hours 12 and 24 respectively following injury （ P 〉 0. 05 ）. Endotoxin： when compared with the conventional and observation groups, statistical significance could also be noted in endotoxin for the control group at hours 12 and 24 following injury （P 〉0.05） , and statistical significance could also be noticed for the observation group at hour 24, when compared with the control and conventional groups （P 〉0.05）. D-lactate： there were statistically significant differences for the conventional group, when comparisons were made between the 3 groups at hour 12 following

关 键 词：海水浸泡 腹部开放伤 肠功能 谷氨酰胺 

分 类 号：R82[医药卫生—临床医学]