Snaill is involved in de novo cardiac fibrosis after myocardial infarction in mice  

作　　者：Yajie Liu Jianlin Du Jin Zhang Minjie Weng Xiaoqun Li Di Pu Lingzhi Gao Songbai Deng Shuang Xia Qiang She 

机构地区：[1]Department of Cardiology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China

出　　处：《Acta Biochimica et Biophysica Sinica》2012年第11期902-910,共9页生物化学与生物物理学报（英文版）

基　　金：Acknowledgement The authors thank Bin Tan from the confocal immuno- fluorescence facility of Pediatric Research Institute of Chongqing Medical University.This work was supported by grants from the National Natural Science Foundation of China （30971213）, the National Natural Science Foundation of China （81100088）,the Important Project of Chongqing Health Administration （20090113）, and the 2010 Key Projects of Chongqing Medical University Foundation （XBZD.201010）.

摘　　要：Epithelial- mesenchymal transition （EMT） is an important mechanism of cardiac fibrosis after myocardial infarction （MI）. However, it remains unclear whether Snail1, an important regulator of EMT, is involved in cardiac fibrosis. In this study, we explored the expression patterns of Snail1 and a cardiac fibrosis marker-- periostin--afler MI in mice and then investigated the co- expression between Snail1 and periostin after MI in mice. Our results showed that the mRNA and protein levels of Snaril1 and perioslin were significantly increased in the infarct area. The Snail1 expression pattern appeared to be parabolic within 14 days after MI. In addition, after MI, all Snail1- positive cells were able to express periostin. These results indi- cate that Snail1 is mainly activated in the infarct area and is involved in de novo cardiac fibrosis after MI in mice. Thus, it is a potential molecular target in the development of drug interventions for ventricular remodeling after MI.Epithelial- mesenchymal transition （EMT） is an important mechanism of cardiac fibrosis after myocardial infarction （MI）. However, it remains unclear whether Snail1, an important regulator of EMT, is involved in cardiac fibrosis. In this study, we explored the expression patterns of Snail1 and a cardiac fibrosis marker-- periostin--afler MI in mice and then investigated the co- expression between Snail1 and periostin after MI in mice. Our results showed that the mRNA and protein levels of Snaril1 and perioslin were significantly increased in the infarct area. The Snail1 expression pattern appeared to be parabolic within 14 days after MI. In addition, after MI, all Snail1- positive cells were able to express periostin. These results indi- cate that Snail1 is mainly activated in the infarct area and is involved in de novo cardiac fibrosis after MI in mice. Thus, it is a potential molecular target in the development of drug interventions for ventricular remodeling after MI.

关 键 词：Snaill PERIOSTIN epithelial-mesenchymaltransition MICE 

分 类 号：Q577[生物学—生物化学] Q813.3