健脾祛瘀法对血脂异常小鼠视网膜色素上皮-玻璃膜-脉络膜毛细血管复合体的影响  

Effects of Jianpi Quyu Decoction on RPE-Bruch's Membrane-Choriocapillaris Complex by the experimental dyslipidemia animal model

作　　者：谢礼丹[1] 曾庆华[1] 莫亚[2] 唐春艳[1] 刘小虎[1] 

机构地区：[1]成都中医药大学眼科教研室,成都610075 [2]成都中医药大学附属医院眼科

出　　处：《中国中医眼科杂志》2012年第5期313-318,共6页China Journal of Chinese Ophthalmology

基　　金：国家自然科学基金资助项目(No.30973774)

摘　　要：目的通过观察健脾祛瘀中药对实验性血脂异常小鼠视网膜色素上皮-玻璃膜-脉络膜毛细血管复合体(retinal pigment epithelium-Bruch’s membrane-choriocapillaris complex,RBCC)的作用,探讨其防治脉络膜新生血管(choroidal neovascularization,CNV)的机理。方法用高脂饲料喂养载脂蛋白E缺乏(apolipoprotein E-deficient,ApoE-/-)小鼠制备血脂异常动物模型,分为高、中、低浓度给药组及阴性对照组,分别给予高浓度、中浓度、低浓度健脾祛瘀中药及生理盐水灌胃1个月。另设普通饲料喂养的普食组。观察各组体质量、血液流变学、血脂的改变;光学显微镜及透射电子显微镜观察RBCC形态及超微结构;免疫组化方法检测RBCC中血管内皮生长因子(vascular endothelial growth factor,VEGF)及其R2受体(vascular endothe-lial growth factor receptor 2,VEGFR2)的表达。结果药物治疗组体质量、血液流变学、血脂指标低于阴性对照组(P<0.05)。普食组视网膜色素上皮(retinal pigment epithelium,RPE)厚度高于阴性对照组和药物治疗组(P<0.05),但药物治疗组与阴性对照组间差异无统计学意义(P>0.05)。各药物治疗组的玻璃膜厚度较阴性对照组明显变薄(P<0.05)。各药物治疗组中VEGF及VEGFR2表达较阴性对照组明显降低(P<0.05)。结论健脾祛瘀中药可减轻实验性血脂异常小鼠RBCC的病理性损害,一定程度上下调RBCC中VEGF、VEGFR2的表达,并可改善模型动物体质量、血脂和血液流变学指标,这可能是其预防和治疗CNV的作用机制。OBJECTIVE To research on the retinal pigment epithelium-Bruch＇s membrane-choriocapillaris complex（RBCC） of the experimental blood lipid abnormality animal model by using Jianpi Quyu Decoction,and to explore the effects in treatment and prevention of CNV.METHODS After high fat feed ordinary human apolipoprotein E-deficient（ApoE-）/-mice set up dyslipidemia animal model and divided into high,medium and low concentration treatment groups.Respectively treated with high,medium and low concentration of Jianpi Quyu Decoction and normal saline gavage for a month.In addition,establish a normal diet group.Observed body quality,blood lipid change of the ordinary feed group,negative control group and the drug treated group.Took off all their eyeballs to make pathological section;Detected VEGF and VEGFR2 expression of RBCC organizations by immunohistochemical method.RESULTS The animal bodymass,lipid and hemorheology levels in the drug treated group were significantly higher than that of the negative control group,the thickness of RPE in the normal forage group was higher than that of other two groups,but there was no difference between treated and negative control group.The Bruch membrane thickness of the treated group was much thicker than the negative control group;Immunity was measured.The treatment group VEGF and VEGFR2 in RBCC was lower compared with the negative control group.CONCLUSIONS Jianpi Quyu Decoction could reduce the experimental dyslipidemia mice bodymass,lipid and hemorheology levels,improve the RBCC pathology organization changes to a certain extent,and down-regulate expression of VEGF and VEGFR2 in RBCC.This may be the mechanism of prevention and treatment of CNV.

关 键 词：健脾祛瘀 血管内皮生长因子 血管内皮生长因子受体2信号通路 年龄相关性黄斑变性 血脂异常 脉络膜新生血管 

分 类 号：R285.5[医药卫生—中药学]