丛枝菌根化枳橙根际微生态环境的研究  

Rhizospheric Niche of Carrizo Citrange Seedlings Colonized by Arbuscular Mycorrhizal(AM) Fungi

作　　者：徐海燕 雷世梅 熊伟 杨灿芳 曾明[4,5] 

机构地区：[1]四川省达州市经济作物推广站,四川达州635000 [2]重庆市农业信息中心,重庆401121 [3]重庆市农业技术推广总站,重庆401147 [4]南方山地园艺学教育部重点实验室,重庆400716 [5]西南大学园艺园林学院,重庆400716

出　　处：《西南大学学报（自然科学版）》2012年第10期65-71,共7页Journal of Southwest University(Natural Science Edition)

基　　金：柑橘模式化栽培与贮藏技术研究(nyhyzx07-023);国家支撑计划"长江柑橘带橙汁加工关键技术研究与产业化开发"(2007BAD47B04)项目基金资助

摘　　要：用根内球囊霉、摩西球囊霉、地表球囊霉及其混合菌剂接种无菌根枳橙幼苗进行盆栽试验,研究接种AM真菌对枳橙幼苗根际土壤微生态环境的影响,结果表明:接种AM真菌的根系形成了40%～70%的菌根侵染率;菌根枳橙的苗高、地茎、地上部/地下部生物量等营养生长显著增加;AM真菌对根际微生物种群数量产生一定的影响,使根区细菌、放线菌、真菌、固氮菌、氨化菌、纤维素分解菌的数量和微生物生物量显著增加(p<0.05);接种AM真菌增加了根际土壤碱性磷酸酶、脲酶的活性,各种酶活性与菌根侵染率呈极显著正相关(p<0.01);接种菌根的根际土壤中,可直接被植物吸收利用的N、P元素出现富集现象,且N、P含量与菌根侵染率呈极显著正相关.通径分析结果表明:枳橙根际土壤微生物、酶活性、pH及养分特性通过直接和间接作用共同影响着菌根真菌的侵染率.In a pot experiment,citrange [Poncirus trifoliate（L.） raf.× Citrus sinensis（L.） Osbeck.] seedlings were inoculated with arbuscular mycorrhizal fungi（AM fungi） of Glomus intraradices,Glomus mosseae,Glomus versiforme and their mixture to study the impact of AM fungi inoculation on rhizosphere microecological environment of the seedlings.An infection rate of mycorrhizal fungi of 40%~80% was obtained in the roots of citrange seedling inoculated with AM fungi.Compared with the control,AM inoculation remarkably increased the vegetative growth of the seedlings,including their height,shoot base diameter and aboveground/belowground biomass.AM fungi inoculation influenced the microbial populations on the roots,and the numbers of bacteria,actinomycetes,fungi,azotobacteria,ammonifiers and cellulose decomposers and microbial biomass in the root zone increased significantly（p0.05）.AM fungi enhanced the activities of alkalinity phosphatase and urease in the soil rhizosphere,and their activities were positively correlated with the infection rate of AM fungi（p0.01）.Enrichment of N and P which could be directly absorbed by the plants occurred in the AM-inoculated rhizosphere soil,and their contents were significantly positively correlated with the mycorrhizal infection rate.Path analysis showed that mycorrhizal infection rate was co-influenced by soil microbes,enzyme activity,pH and nutrient contents in the rhizosphere through their direct or indirect actions.

关 键 词：丛枝菌根 枳橙 根际土壤 土壤养分 土壤酶活性 通径分析 

分 类 号：Q938.1[生物学—微生物学]