DC-SIGN和DC-SIGNR基因多态性分析与HIV-1易感性关系研究  

Correlation between polymorphisms of DC-SIGN and SIGNR gene and susceptibility of HIV-1

作　　者：陈绮珊[1] 李燕[1] 徐慧芳[1] 梁彩云[1] 高凯[1] 韩志刚[1] 罗碧莲[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510440

出　　处：《热带医学杂志》2012年第10期1181-1183,1194,共4页Journal of Tropical Medicine

基　　金：广东省医药卫生科技项目(A2009563);广州市科技项目(2006ZI-E0091);广州市医药卫生科技项目(2007-ZDi-08)

摘　　要：目的通过分析DC-SIGN和DC-SIGNR的基因多态性分布及基因频率在健康人群、HIV-1感染者和HIV-1长期暴露不感染人群(ES)的差别,探讨这两个基因与HIV-1长期暴露不感染的关系。方法纳入三组研究对象,包括健康对照组(160例),HIV-1感染者组(267例),ES组(37例)。扩增DC-SIGN和DC-SINGR基因的颈区重复序列进行分析。结果三组人群DC-SIGN基因型以7/7为主,等位基因呈低度多态性。DC-SIGNR基因型以7/7为主,等位基因呈高度多态性,出现了较多的非7/7基因型,其中等位基因6在三组间以及在HIV-1感染者和ES之间分布的差异均有统计学意义(P<0.05)。结论 DC-SIGN基因多态性变异很低,对人群HIV-1易感性的研究意义不大;DC-SIGNR基因呈高度多态性,其低频率等位基因6可能是人群对HIV-1不易感的保护因素。Objective To explore the correlation of low susceptibility to HIV-1 with gene polymorphism of DC-SIGN and DC-SIGNR. Methods One hundred and sixty normal controls, 267 HIV-1 infected individuals and 37 ES were included in the study. The repeated sequences of DC-SIGN and DC-SINGR were amplificated by PCR and analyzed. Results Genotype 7/7 was the primary genotype of DC-SIGN alleles showed low polymorphism among the three groups. Genotype 7/7 was the primary genotype of DC-SIGNR and more non 7/7 genotypes were identified among the three groups. The analysis of polymorphism information content showed that DC-SIGNR alleles had high polymorphism. The frequency of allele 6 of ES people was lower than that of HIV-1 infected individuals （P0.05） and normal controls （P0.05）. Conclusions The present study showed that DC-SIGN alleles had low polymorphism in Chineses and it has little research significance. DC-SIGNR alleles had high polymorphism in Chinese and the low frequency of allele 6 may be correlated with low susceptibility to HIV-1.

关 键 词：HIV-1 暴露 不感染 基因多态性 DC-SIGN DC-SIGNR 

分 类 号：R512.91[医药卫生—内科学]