阿霉素对K562细胞凋亡及FAK mRNA的影响  

Effects of ADM on cell apoptosis and expression of FAK mRNA in K562

作　　者：王英[1] 许吕宏[1] 段朝晖[1] 曹开源[1] 罗金刚[1] 许英[1] 史沛杰[1] 

机构地区：[1]中山大学孙逸仙纪念医院检验科,广东广州510120

出　　处：《热带医学杂志》2012年第10期1195-1198,共4页Journal of Tropical Medicine

基　　金：中央高校基本科研业务费专项基金(09ykpy08)

摘　　要：目的探讨阿霉素体外作用于K562细胞后,观察其对细胞增殖的影响,促进细胞凋亡情况,以及调节细胞周期和粘着斑激酶(FAK)mRNA基因,进一步探讨通过调控FAK表达,研究抗白血病的作用机制。方法应用细胞增殖实验(CCK8法)观察不同浓度阿霉素作用不同时间对K562细胞增殖的影响,应用流式细胞仪观察不同浓度阿霉素对K562细胞细胞凋亡,细胞周期的影响,应用RT-PCR和Western blot技术检测不同浓度阿霉素作用对K562细胞36h后FAK mRNA以及蛋白表达水平的变化。结果随着阿霉素浓度增加及作用时间延长,K562细胞的增殖抑制率逐渐升高,同一时间不同浓度之间比较,或者同一浓度不同时间组之间比较,差异均有统计学意义(P<0.05);阿霉素能引起K562细胞凋亡,且随着药物浓度增加,凋亡率也逐渐增加,差异均有统计学意义(P<0.05);阿霉素能引起K562细胞周期阻滞,多停留在S期;阿霉素能引起K562细胞FAK mRNA表达显著降低。阿霉素能引起K562细胞FAK蛋白表达水平的降低。结论阿霉素抑制分裂期细胞的增殖,诱导细胞凋亡增加,对细胞FAK基因和蛋白水平均显著下调,为进一步研究FAK基因表达的调控与肿瘤细胞凋亡的分子机制提供了实验基础。Objective To investigate the effects of ADM on proliferation,cell cycle progression,apoptosis and the expression of focal adhesion kinase（FAK） mRNA in human chronic myeloid leukemia （CML） cell line K562, and further to explore the mechanism of regulation of FAK. Methods The CCK8 method was used to detect the effects of ADM on the proliferation of K562 cells; flow cytometry was used to determine the effects of ADM on the cell cycle progression and apoptosis of K562 cells. RT-PCR and Western blot was used to assay the expression of FAK mRNA and FAK protein in K562 cells treated with ADM for 36 hours. Results ADM exhibit a dose- and time-dependent inhibition of K562 proliferation, and the effect was significant （P0.05）. ADM could also significantly induce apoptosis of K562 cells （P0.05）. ADM arrested K562 cell at S phase. ADM could decrease the expression levels of FAK mRNA and FAK proteins in K562 cells. Conclusions ADM is effective to reduce the expression of FAK mRNA and FAK protein, and to induce cell cycle arrest and apoptosis in K526 cells. The results may provide the research basis for further study of the role of FAK in tumour cell apoptosis.

关 键 词：K562细胞 阿霉素 细胞增殖 凋亡 FAK MRNA 

分 类 号：Q813.5[生物学—生物工程]