RNAi screening of the human colorectal cancer genome identifies multifunctional tumor suppressors regulating epithelial cell invasion  

作　　者：PeterLy  UgurEskiocak  Chelsea RParker  Kenneth JHarris  Woodring EWright Jerry WShay 

机构地区：[1]Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA[2]Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia

出　　处：《Cell Research》2012年第11期1605-1608,共4页细胞研究（英文版）

摘　　要：Dear Editor, Genomic sequencing of human tumor-derived DNA has identified a vast number of novel tissue-specific genetic mutations occurring at varying frequencies [1]. Frequently mutated genes are informally referred to as driver alterations （or candidate cancer genes, CAN- genes）, whereas those less frequently mutated are con- sidered passenger alterations [ 1, 2]. With few exceptions, the majority of mutations are loss-of-function alterations and the ones that occur rarely are presumed to be a by- product of genomic instability or normal mutation rates that do not directly contribute to tumor progression （pas- sengers） [3]. In addition to computational and biostatisti- cal models for parsing driver from passenger mutations, the utility of biological assays to functionally interrogate cancer genomes is required to determine which of these mutations are able to contribute to a particular tumori- genic phenotype （i.e., anchorage-independent growth, resistance to apoptosis, enhanced invasion through extra- cellular matrices）.

关 键 词：肿瘤抑制基因 基因组测序 RNAi 上皮细胞 人类 多功能 大肠癌 驱动程序模型 

分 类 号：Q78[生物学—分子生物学] Q987