电泳法与高压液相亲和层析法测定HbA1c的方法学比较  

The electrophoresis method and high performance liquid chromatography in glycosylated hemoglobin A1c determination:A methodological comparison

作　　者：赵静华[1] 张丽坤[1] 桂晓巍[1] 陈冬梅[1] 

机构地区：[1]辽宁医学院附属第三医院检验科,辽宁锦州121000

出　　处：《中国现代医生》2012年第31期107-108,共2页China Modern Doctor

摘　　要：目的对电泳法测定HbA1c进行方法学评价。方法收集56份标本分别用高压液相亲和层析法(HPLC)和电泳法检测。按照HPLC法检测结果将样本分为低、中、高三组,再使用电泳法测定。结果低值组中两种方法检测结果分别为(6.31±0.32)和(6.11±0.41),差异有统计学意义(P<0.05),相关分析Y=0.94X+0.73(r=0.940,r2=0.884);中值组中两种方法检测结果分别为(8.49±0.75)和(8.35±0.77),差异有统计学意义(P<0.05),相关分析Y=0.96X+0.94(r=0.962,r2=0.925);高值组中两种方法检测结果分别为(12.77±1.77)和(12.69±1.70),差异无统计学意义(P>0.05)。结论电泳法具有较好的精密度,两种方法相关性较好。Objective To evaluate the electrophoresis method for determination of glycosylated hemoglobin A lc （HbAlc）. Methods A total of 56 specimens were detected respectively with High performance liquid chromatography （HPLC） and electrophoresis method. Results Samples were divided into there groups：high, medium and low value gropus according to the results value of the HPLC. results of low value groups were respctively （6.31±0.32）with HPLC and （6.11 ±0.41） with electrophoresis method and the difference was statistically significant （P 〈 0.05 ） and there was correlation between two methods （Y = 0.94X±0.73, r = 0.940, r2 = 0.884）; results of medium value groups were respctively （8.49±0.75） with HPLC and （8.35±0.77） with electrophoresis method and the difference was statistically significant （P 〈 0.05）, there was correlation between two methods （Y = 0.96X±0.94, r = 0.962, r2 = 0.925）; results of high value groups were（12.77±l.77） with HPLCand （12.69±1.70） with electrophoresis method and there was no statistical significance between two methods. Conclusion Electrophoresis method has good precision, linear and anti-interference; statistics analysis indicate that the minimum and median values of the two methods have statistical significance, the correlation between the two methods is good.

关 键 词：糖化血红蛋白 电泳法 高压液相亲和层析法 方法学 

分 类 号：R446.112[医药卫生—诊断学] R587.1[医药卫生—临床医学]