PEPCK-C^(mus)转基因小鼠的表型鉴定  

Analysis of Phenotype of PEPCK-C^(mus) Transgenic Mice

作　　者：冯玮[1] 任媛媛[2] 刘乃丰[1] 任子健[2] 于明[2] 程俊霖[2] 王盈[2] 朱洁[2] 金丹[2] 杨海元[2] 郜倩倩[2] 李倩坤[2] 戴一凡[2] 

机构地区：[1]中国药科大学药学院,南京210029 [2]南京医科大学代谢疾病研究中心,南京210029

出　　处：《生物物理学报》2012年第10期815-825,共11页Acta Biophysica Sinica

基　　金："973"计划项目(2009CB918904);国家自然科学基金项目(81070671)~~

摘　　要：磷酸烯醇式丙酮酸羧激酶(phosphoenolpyruvate carboxykinase,PEPCK)是催化糖异生的限速酶,其在骨骼肌中表达量很低,作用尚不明确。本研究构建了在骨骼肌中特异表达PEPCK-C的转基因小鼠(PEPCK-Cmus小鼠),探讨PEPCK-C在小鼠骨骼肌中特异性表达后对运动和能量代谢的影响。结果显示,与同龄的野生型小鼠比较,PEPCK-Cmus转基因小鼠运动能力更强,胰岛素敏感性更高,血脂水平较低;骨骼肌中有明显脂肪堆积,但空腹血糖、体重、腹部脂肪体积没有差异。结果表明,PEPCK-C基因在小鼠骨骼肌中的特异性表达,提高了小鼠的运动耐力和糖平衡能力,并使血脂维持在较低水平,提示PEPCK-C基因在骨骼肌中的表达对机体的能量代谢有重要作用。Phosphoenolpyruvate carboxykinase （PEPCK） is a rate-limiting enzyme for catalyzing gluconeogenesis, which is rarely expressed in skeletal muscle, but the role of PEPCK-C in muscle is not clear. Somatic mutagenesis was used to selectively over-express PEPCK-C gene in skeletal muscle of mice in this study. Compared with wild type mice （WT mice）, PEPCK-C＇~ mice had an enhanced exercise capacity, increased insulin sensibility, and maintained lower serum triglyceride. More fat accumulation in skeletal muscle was observed in PEPCK-C~ mice than in controls, while there was no difference of fasting blood glucose, body weight and distribution and size of ventral adipose between PEPCK-C＇~ mice and WT mice. Our results indicateded that PEPCK-C gene had important physiological effects on exercise tolerance and adjusting blood glucose balance and keep serum triglyceride at low level, therefore PEPCK-C gene in muscle is important for system metabolism.

关 键 词：磷酸烯醇式丙酮酸激酶 骨骼肌 胰岛素敏感性 糖代谢 

分 类 号：Q789[生物学—分子生物学]