DJB术对2型糖尿病大鼠肠神经GLP-1受体表达影响的研究  

Effects of duodenal jejunal bypass on the expression of GLP-1R in the enteric nervous system of type 2 diabetic rats

作　　者：张秀荣[1,2] 徐继马[1,3] 严庆涛[1,4] 季万胜[3] 房春燕[1,2] 王琳[1,2] 赵廷坤[1,2] 高志芹[1,5] 曲梅花[1,2] 

机构地区：[1]潍坊医学院山东省高校应用药理学重点强化建设实验室,山东潍坊261053 [2]潍坊医学院药理学教研室,山东潍坊261053 [3]潍坊医学院附属医院消化内科,山东潍坊261031 [4]潍坊市人民医院小儿外科,山东潍坊261041 [5]潍坊医学院细胞生物学教研室,山东潍坊261053

出　　处：《山东大学学报（医学版）》2012年第11期1-5,共5页Journal of Shandong University:Health Sciences

基　　金：国家教育部留学回国人员科研启动基金(2011年第43批);山东省自然科学基金(ZR2009DM024;ZR2011HM043;2009ZRB019GN;ZR2009CL047);山东省教育厅科研基金(J11LF33)

摘　　要：目的研究十二指肠空肠旁路手术(duodenal jejunal bypass,DJB)对2型糖尿病(T2DM)大鼠肠神经细胞胰高血糖素样肽-1受体(glucagon-like peptide-1 receptor,GLP-1R)表达的影响,探讨DJB手术后肠神经GLP-1R表达、血清GLP-1分泌变化与血糖的关系。方法雄性Wistar大鼠随机分为4组,每组8只:Wistar大鼠对照组(W-C);Wistar大鼠DJB手术组(W-DJB);T2DM模型对照组(T2DM-C);T2DM+DJB手术组(T2DM-DJB)。分别测定各组大鼠术前及术后第1、4、8周空腹血糖、空腹GLP-1,术前及术后第8周空腹胰岛素水平。术后第8周处死大鼠,免疫荧光、RT-PCR检测回肠末端肠肌间神经丛GLP-1R表达变化。结果 T2DM-DJB组大鼠肠神经丛表达GLP-1R的细胞数目为15.83±2.23,明显高于T2DM-C组大鼠表达GLP-1R的细胞数目8.50±2.67(P<0.01);GLP-1R mRNA在T2DM-DJB组大鼠肠神经丛表达水平为0.67±0.08,明显高于T2DM-C组大鼠GLP-1RmRNA的表达水平0.42±0.03(P<0.01);T2DM-DJB组大鼠术后空腹血糖、空腹胰岛素显著降低(P<0.01),空腹血清GLP-1显著升高(P<0.01)。W-DJB组大鼠术后GLP-1R表达水平、空腹血糖、空腹胰岛素和空腹血清GLP-1与W-C组大鼠相比差异无统计学意义(P>0.05)。结论 DJB手术能显著降低T2DM大鼠血糖,对正常大鼠无明显作用。Objective To study the effects of duodenal jejunal bypass（DJB） on the expression of Glucagon-like peptide-1receptor（GLP-1R） in the enteric nervous system of type 2 diabetic（T2DM）rats,and to explore the relationship between GLP-1R expression,GLP-1 secretion and blood glucose.Methods Wistar rats were randomly divided into four groups： Wistar control group（W-C）,Wistar DJB surgery group（W-DJB）,T2DM control group（T2DM-C）,T2DM DJB surgery group（T2DM-DJB）,8 rats in each group.Fasting blood glucose and glucagon-like peptide-1（GLP-1） were detected before DJB operation and 1week,4 weeks,8 weeks after DJB operation.Meanwhile,fasting insulin before DJB operation and 8 weeks after DJB operation was also detected.After 8 weeks,the rats were sacrificed and the expression of GLP-1R in the enteric nervous was studied by RT-PCR and immunofluorescence.Results The number of GLP-1R positive cells in the T2DM-DJB rats enteric nervous system was 15.83±2.23,it increased significantly compared to that of the T2DM-C rats which was 8.50±2.67（P0.01）.The expression of GLP-1R mRNA in the T2DM-DJB rats enteric nervous system was 0.67±0.08,it also increased significantly compared to that of the T2DM-C rats which was 0.42±0.03（P0.01）.The fasting blood glucose and fasting insulin of T2DM-DJB rats decreased after DJB surgery（P0.01）,and the fasting serum GLP-1 increased after DJB surgery（P0.01）.GLP-1R expression level,fasting blood glucose,fasting insulin and fasting serum GLP-1 of the W-DJB rats had no significant difference after DJB surgery compared to those of the W-C rats（P0.05）.Conclusion DJB surgery can significantly decrease the fasting blood glucose of T2DM rats,but it has no obvious effect in normal rats.

关 键 词：十二指肠空肠旁路术 糖尿病 2型 肠神经系统 胰高血糖素样肽-1受体 

分 类 号：R587.1[医药卫生—内分泌]