血管扩张刺激磷蛋白与微管蛋白的结合  

The combination between vasodilator-stimulated phosphoprotein and microtubule protein

作　　者：陶燕[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《山东大学学报（医学版）》2012年第11期18-22,29,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金青年项目(31100974);江苏大学高级人才启动基金(11JDG032)

摘　　要：目的探讨血管扩张刺激磷蛋白(VASP)及其丝氨酸157(p-VASP S157)和239(p-VASP S239)位点磷酸化与α-微管蛋白(α-Tubulin)之间的关系,明确VASP是一个新的微管相关蛋白。方法采用免疫荧光染色观察p-VASP S157、p-VASP S239和α-Tubulin在人宫颈癌上皮细胞HeLa的共定位;利用免疫共沉淀、微管沉淀和Western blot方法明确VASP及其不同位点磷酸化与α-Tubulin的结合情况。结果 VASP和p-VASP S157能够与α-Tubulin结合;p-VASP S239在细胞周期各个时期与α-Tubulin均无共定位关系。结论 VASP是一个新的微管相关蛋白,其丝氨酸157磷酸化与二者的结合相关。Objective To investigate the relationship between vasodilator-stimulated phosphoprotein（VASP）,serine 157/serine 239 phosphorylated VASP（p-VASP S157/p-VASP S239） and α-Tubulin,and to confirm VASP as a new microtubule-associated protein（MAP）.Methods The co-localization of p-VASP S157/p-VASP S239 and α-Tubulin in Hela cells were analyzed by the immunofluorescencent assay.Co-immunoprecipitation assay,Microtubule pelleting assay and Western blot assay were applied to detect the combination between VASP/phosphor-VASP and α-Tubulin.Results VASP and p-VASP S157 could bind with α-Tubulin.p-VASP S239 did not co-localize with α-Tubulin during the whole cell cycle.Conclusion VASP is a new microtubule-associated protein,and the serine 157 phosphorylation of the protein is involved in the association between VASP and α-Tubulin.

关 键 词：血管扩张刺激磷蛋白 磷酸化血管扩张刺激磷蛋白 Α-微管蛋白 微管相关蛋白 人宫颈癌上皮细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]