Ⅰ/Ⅲ型胶原膜联合自体骨髓间充质干细胞修复兔膝关节软骨缺损  

Repairing articular cartilage defects in the knee of rabbits using type Ⅰ/Ⅲ-collagen-membrane combined with autologous bone marrow mesenchymal stem cells

作　　者：贺冬冬[1] 曾令员[1] 向川[1] 王宇泽[1] 王世东[1] 段王平[1] 吕嘉[1] 王春芳[1] 卫小春[1] 

机构地区：[1]山西医科大学第二医院骨与软骨损伤修复重点实验室,山西省太原市030001

出　　处：《中国组织工程研究》2012年第38期7031-7036,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究表明Ⅰ型胶原和Ⅲ型胶原都有利于骨髓间充质干细胞的黏附、增殖和分化。目的:观察Ⅰ/Ⅲ型胶原膜联合自体骨髓间充质干细胞修复兔膝关节软骨缺损的可行性。方法:取24只新西兰大白兔制作双膝股骨滑车直径3.8mm、深2mm关节软骨缺损模型,分为2组:实验组缺损区植入Ⅰ/Ⅲ型胶原膜-自体骨髓间充质干细胞复合物,对照组缺损区植入单纯Ⅰ/Ⅲ型胶原膜。结果与结论:膝关节股骨标本组织学染色显示,实验组植入8周时为类透明软骨修复,12周时接近于正常软骨;对照组植入8周时以纤维样组织修复为主,12周时为纤维软骨修复。实验组植入后8,12周组织学评分均明显高于对照组(P<0.001)。表明运用Ⅰ/Ⅲ型胶原膜-自体骨髓间充质干细胞复合物可修复关节软骨缺损。BACKGROUND：Several researches have shown that type Ⅰand type Ⅲ collagens are conducive to the adhesion,proliferation and differentiation of bone marrow mesenchymal stem cells.OBJECTIVE：To investigate the feasibility of repairing articular cartilage defects in the knee of rabbits using autologous bone marrow mesenchymal stem cells combined with the type Ⅰ/Ⅲ-collagen-membrane.METHODS：Totally 24 New Zealand rabbits were randomly divided into two groups.A model of full-thickness articular cartilage defects in the bilateral knees with 3.8 mm in diameter and 2 mm in depth was established.The defects filled with the compound of autologous bone marrow mesenchymal stem cells/collagen membrane in 12 rabbits were as the experimental group,while the defects filled with collagen membrane only in another 12 rabbits were as the control group.RESULTS AND CONCLUSION：The histological staining for the knee femoral specimens showed that the repair tissues mainly were hyaline-like cartilage at week 8,and close to the normal cartilage at week 12 in the experimental group.However,in the control group,the repair tissues mainly were fibrous tissues at week 8,and fiber-based cartilage was repaired at week 12.At 8 and 12 weeks after implantation,the histological scores of the experimental group were significantly higher than those of the control group （P 0.001）.These findings suggest that it is a good way for autologous bone marrow mesenchymal stem cells-Ⅰ/Ⅲ-collagen-membrane to repair articular cartilage defects.

关 键 词：Ⅰ/Ⅲ型胶原膜 骨髓间充质干细胞 关节软骨缺损 修复 兔 生物材料 

分 类 号：R318[医药卫生—生物医学工程]