纳米羟基磷灰石/聚己内酯复合大鼠骨髓间充质干细胞的生物相容性  

Biocompatibility of nano hydroxyapatite/polycaprolactone compounded with rat bone marrow mesenchymal stem cells

作　　者：李家锋[1] 徐金霞[2] 管海虹[3] 崔群[2] 孙秀英[2] 陈丽娟[3] 韩建国[2] 

机构地区：[1]徐州医学院附属医院口腔颌面外科,江苏省徐州市221002 [2]徐州医学院口腔医学院,江苏省徐州市221004 [3]徐州医学院附属徐州市立医院口腔颌面外科,江苏省徐州市221002

出　　处：《中国组织工程研究》2012年第38期7042-7046,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:纳米羟基磷灰石/聚己内酯是一种具有优良生物相容性和生物活性的典型生物复合材料。目的:分析纳米羟基磷灰石/聚己内酯电纺薄膜作为组织工程骨支架的可行性。方法:采用静电纺丝技术制备纳米羟基磷灰石/聚己内酯电纺薄膜,将其与第3代SD大鼠骨髓间充质干细胞复合培养,在地塞米松、β-磷酸甘油钠、维生素C成骨诱导剂诱导下,诱导骨髓间充质干细胞向成骨细胞转化。结果与结论:纳米羟基磷灰石/聚己内酯支架具有合适的微孔结构,且孔道相互贯通。①倒置显微镜观察:复合培养7d后细胞大部分为梭形,细胞开始分裂;14d后,细胞生长比较旺盛,数量明显增多,细胞分泌基质并黏附于支架上。②扫描电镜观察:复合培养7d后大量细胞位于支架孔隙内生长,增殖良好,细胞大多呈梭形,双极突起,形态较佳,呈立体状生长,并分泌基质,有纤维连接蛋白生成。表明纳米羟基磷灰石/聚己内酯支架具有良好的生物相容性,是骨组织工程的良好载体。BACKGROUND：Nano hydroxyapatite/polycaprolactone with excellent biocompatibility and biological activity is a typical biological compound material.OBJECTIVE：To investigate the feasibility of nano hydroxyapatite/polycaprolactone electrospun film as scaffold materials for bone tissue engineering METHODS：First,nano hydroxyapatite/polycaprolactone electrospun film was prepared by electrostatic spinning technology.Second,the nano hydroxyapatite/polycaprolactone electrospun film was co-cultured in vitro with passage 3 bone marrow mesenchymal stem cells of rats.Then,bone marrow mesenchymal stem cells were induced into osteoblasts by dexamethasone,β-glycerophophate and vitamin C.RESULTS AND CONCLUSION：Nano hydroxyapatite/polycaprolactone scaffolds possessed good macro porous structure,and pores were interconnected with each other.①Inverted microscope observation：After bone marrow mesenchymal stem cells and the scaffold were co-cultured for 7 days,cells were mostly fusiform and began to proliferate.After 14 days,cell growth was more vigorous,and the number of cells was increased significantly.Moreover,cells secreted the extracellular matrix and adhered to the scaffold.②Scanning electron microscope observation：At day 7,a large number of cells grew in scaffold pores,and they had active proliferation capacity.Besides,cells were mostly fusiform,and had good bipolar protrusions.In addition,the cells showed three-dimensional-like growth and secreted the extracellular matrix,as well as fibronectin was produced.These results suggest that the nano hydroxyapatite/polycaprolactone scaffold has good biocompatibility,and it can be used as a good carrier for bone tissue engineering.

关 键 词：纳米羟基磷灰石 聚己内酯支架 骨髓间充干质细胞 诱导成骨 生物相容性 电纺薄膜 

分 类 号：R318[医药卫生—生物医学工程]