高浓度纳米羟基磷灰石凝胶对Hela肿瘤细胞的作用  

Effect of high-concentration nano hydroxyapatite sol on Hela cells

作　　者：卞铁荣[1] 邢宏运[1] 卢正欣[2] 严富学[2] 陈庄[1] 陈枫[1] 

机构地区：[1]泸州医学院附属医院医学实验中心,四川省泸州市646000 [2]西安理工大学现代分析检测中心,陕西省西安市710054

出　　处：《中国组织工程研究》2012年第38期7063-7067,共5页Chinese Journal of Tissue Engineering Research

基　　金：泸州医学院附属医院提供的资金资助

摘　　要：背景:有文献质疑羟基磷灰石凝胶浓度越高杀死癌细胞的能力越强。目的:观察不同质量浓度纳米羟基磷灰石凝胶对Hela肿瘤细胞的杀伤作用。方法:采用化学沉淀法制备5,10,20,40,80,160,320,640mg/L不同质量浓度的纳米羟基磷灰石凝胶,分别加入Hela细胞中,以在Hela细胞中加入5,10mg/L紫杉醇为对照。结果与结论:①不同质量浓度纳米羟基磷灰石凝胶对Hela肿瘤细胞均有杀伤作用,且随着质量浓度的提高,杀伤作用也逐渐提高,但其对肿瘤细胞的生长抑制作用不及紫杉醇。②纳米羟基磷灰石凝胶与紫杉醇对Hela肿瘤细胞杀伤作用在第2天有所下降,第3天又呈现提高趋势,且第3天的抑制作用较第1天更明显,并且所有质量浓度的纳米羟基磷灰石凝胶均呈现这个趋势。③纳米羟基磷灰石凝胶对Hela肿瘤细胞的生长有一定抑制作用,紫杉醇作用后细胞不呈现明显增殖,纳米羟基磷灰石凝胶作用后细胞有不同程度增殖。BACKGROUND：Several literatures have questioned that with the increase of nano hydroxyapatite sol concentration,the capacity of killing tumor cells becomes stronger.OBJECTIVE：To investigate the killing effect of nano hydroxyapatite sol with different concentrations on Hela cells.METHODS：Nano hydroxyapatite sol with the concentrations of 5,10,20,40,80,160,320 and 640 μg/mL were synthesized by the chemical precipitation method and added into the Hela cells.The Hela cells added with 5 and 10 mg/L taxol served as controls.RESULTS AND CONCLUSION：①Nano hydroxyapatite sol with different concentrations had killing effect on Hela cells;as the concentration of nano hydroxyapatite sol increased;the killing effect was gradually improved.But their inhibited effect was not better than that of taxol.②The killing effect of nano hydroxyapatite sol and the taxol on Hela cells began decreased at day 2,but it increased in the following day.The inhibited effect at day 3 was more obvious than that at day 1,and nano hydroxyapatite sol with various concentrations were all showed this tendency.③A certain inhibited effect of nano hydroxyapatite sol on Hela cells was found.The proliferation of Hela cells was not significant after given taxol,while Hela cells were found proliferation in various degrees after given nano hydroxyapatite sol.

关 键 词：纳米羟基磷灰石 HELA细胞 紫杉醇 化学沉淀法 细胞毒性 肿瘤细胞 抑制 生物材料 

分 类 号：R318[医药卫生—生物医学工程]