静电纺丝聚乳酸聚乙醇酸共聚物-丝素-胶原神经导管修复大鼠周围神经缺损  

Repairing peripheral nerve defects in rats by electrostatic spinning poly (lactic-co-glycolic acid)-silk fibroin-collagen nerve conduits

作　　者：李森[1] 史晓远[1] 林卫[2] 范红松[3] 陈宝[1] 吴晖[1] 胡旭栋[1] 王光林[1] 

机构地区：[1]四川大学华西医院骨科,四川省成都市610041 [2]四川大学华西妇产儿童医院,四川省成都市610041 [3]四川大学生物材料中心,四川省成都市610041

出　　处：《中国组织工程研究》2012年第38期7087-7091,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30772204);四川省科技支撑计划项目(2100FZ0020)~~

摘　　要：背景:周围神经缺损修复是临床上一大难题,由于自体神经移植有一定的局限性,人工神经修复材料是一种很有前途的选择。目的:探讨静电纺丝聚乳酸聚乙醇酸共聚物(PLGA)-丝素-胶原纳米神经导管修复大鼠坐骨神经缺损的可能性。方法:雌性SD大鼠36只,制备约10mm的坐骨神经缺损,分别采用倒转自体神经、静电纺丝PLGA-丝素-胶原神经导管、单纯PLGA神经导管桥接,术后12周进行大体观察、神经电生理测定、光镜观察、透射电镜观察和图像分析对比,了解神经再生的情况。结果与结论:静电纺丝法制备成的纳米神经导管管壁疏松多孔,能够模拟细胞外基质的结构。静电纺丝PLGA-丝素-胶原神经导管组在促进坐骨神经再生、提高再生神经髓鞘化、加速再生神经功能重建等方面均优于单纯PLGA导管组,比自体神经移植组略差。BACKGROUND：The repair of peripheral nerve defects exhibits a clinical challenge.Because of the limitation of nerve autograft,it is a promising choice for artificial nerve grafts.OBJECTIVE：To investigate the possibility of repairing sciatic nerve defects by electrostatic spinning poly （lactic-co-glycolic acid） （PLGA）-silk fibroin-collagen nano nerve conduits.METHODS：Totally 36 female SD rats were underwent 10 mm sciatic nerve defects.The nerves of rats were grafted by reverse nerve autografts,electrostatic spinning PLGA-silk fibroin-collagen nerve conduits and PLGA nerve conduits alone.At week 12 postoperatively,nerve regeneration was studied by general observations,electrophysiological examination,microscopic observation,transmission electron microscopy observation and image analysis.RESULTS AND CONCLUSION：The results showed that the nanofibers-based nerve conduits prepared by electrostatic spinning had a well-distributed porous structure and could simulate the structure of native extracellular matrix.These findings suggest that the electrostatic spinning PLGA-silk fibroin-collagen nerve conduits group is superior to PLGA nerve conduits group in sciatic nerve regeneration,regenerative nerve myelinization and nerve foundation reconstruction,but slightly worse than the nerve autograft group.

关 键 词：静电纺丝 神经导管 组织工程 神经再生 聚(乳酸-乙醇)酯共聚物 丝素 胶原 生物材料 

分 类 号：R318[医药卫生—生物医学工程]