微弧氧化AZ31镁合金的生物相容性  

Biocompatibility of AZ31 magnesium alloy treated with micro-arc oxidation

作　　者：王树峰[1] 李春荣 王程越[3] 李淑华[4] 谭丽丽[5] 杨柯[5] 

机构地区：[1]辽宁医学院研究生学院,辽宁省锦州市121000 [2]新疆裕民县人民医院口腔科,新疆维吾尔自治区裕民县834800 [3]辽宁医学院第五临床医院,辽宁省锦州市121000 [4]辽宁医学院免疫与微生物学实验室,辽宁省锦州市121000 [5]中国科学院金属研究所,辽宁省沈阳市110016

出　　处：《中国组织工程研究》2012年第38期7101-7106,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省教育厅课题资助项目(2009T063)~~

摘　　要：背景:表面改性技术和合金成分的变化都会影响到镁合金的生物特性。目的:探讨微弧氧化处理AZ31镁合金的生物相容性。方法:将体外扩增培养的兔第3代骨髓基质细胞接种到未处理AZ31镁合金(镁合金+骨髓基质细胞组)和微弧氧化AZ31镁合金(微弧氧化镁合金+骨髓基质细胞组)材料上,另设骨髓基质细胞组做对照。培养1,3,5,7d观察细胞形态,细胞生长状况,细胞黏附状况,材料表面状况。结果与结论:3组细胞形态一致,以梭形为主。镁合金+骨髓基质细胞组中细胞生长状况明显不如微弧氧化镁合金+骨髓基质细胞组和骨髓基质细胞组(P<0.05)。微弧氧化镁合金+骨髓基质细胞组材料表面有大量的细胞附着,并且生长正常,镁合金+骨髓基质细胞组材料表面没有细胞附着,有明显的裂痕(P<0.05)。结果证实,微弧氧化处理AZ31镁合金具有良好的生物相容性,有利于细胞的附着和正常生长。BACKGROUND：Surface modification and the alteration of alloying components will affect the biocompatibility of magnesium alloy.OBJECTIVE：To evaluate the biocompatibility of AZ31 magnesium alloy treated with micro-arc oxidation.METHODS：The passage 3 rabbit bone marrow stroma cells （BMSCs） cultured and expanded in vitro were collected.The cells were implanted into the un-treated AZ31 magnesium alloy （magnesium alloy＋BMSCs group） and AZ31 magnesium alloy treated with micro-arc oxidation （magnesium alloy treated with micro-arc oxidation＋BMSCs group）.In addition,BMSCs were as the blank control group （BMSCs group）.Cellular morphology,cell growth and adhesion conditions as well as material surface appearance were observed after cultured for 1,3,5 and 7 days.RESULTS AND CONCLUSION：The cellular morphology in each group was the same,and mainly showed fusiformis.The cell growth condition of the magnesium alloy＋BMSCs group was significantly not better than that of magnesium alloy treated with micro-arc oxidation＋BMSCs group and BMSCs group （P 0.05）.A large number of cells that grew normally were found adhesion in the material surface of the magnesium alloy treated with micro-arc oxidation＋BMSCs group.There were no cells adhered onto the material surface and obvious cracks were seen in the magnesium alloy＋BMSCs group.These findings suggest that AZ31 magnesium alloy treated with micro-arc oxidation has good biocompatibility,which is helpful to cell adhesion and normal growth.

关 键 词：微弧氧化 AZ31镁合金 骨髓基质细胞 生物相容性 组织工程 

分 类 号：R318[医药卫生—生物医学工程]