应用环磷酰胺构建C57BL/6J小鼠免疫抑制模型  

Establishing a C57BL/6J mouse immunosuppressive model induced by cyclophosphamide

作　　者：杨宪勇[1] 

机构地区：[1]泰安市中心医院,山东省泰安市271000

出　　处：《中国组织工程研究》2012年第40期7486-7490,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:在免疫增强剂的研究中,常用到免疫抑制模型,如何建立免疫抑制模型成为免疫增强剂作用评价的关键。目的:应用环磷酰胺构建C57BL/6J小鼠免疫抑制模型。方法:将小鼠随机分为正常对照组、环磷酰胺50mg/kg5d组,环磷酰胺80mg/kg3d组,环磷酰胺80mg/kg5d组,环磷酰胺100mg/kg5d组和环磷酰胺100mg/kg隔天给药组。正常对照组小鼠腹腔注射生理盐水0.1mL,连续5d。环磷酰胺50mg/kg5d组,环磷酰胺80mg/kg3d组,环磷酰胺80mg/kg5d组和环磷酰胺100mg/kg5d组小鼠分别以环磷酰胺50,80,80,100mg/kg腹腔注射连续5,3,5,5d。环磷酰胺100mg/kg隔天给药组小鼠腹腔注射100mg/kg环磷酰胺,隔天1次,共注射3次。结果与结论:与正常对照组比较,腹腔注射环磷酰胺可导致小鼠外周血中CD3+T,CD3+CD4+T及CD3+CD8+T细胞明显下降(P<0.05);谷丙转氨酶(除环磷酰胺50mg/kg5d、80mg/kg3d组)、谷草转氨酶、尿素显著升高(P<0.05),其中环磷酰胺80mg/kg5d组、环磷酰胺100mg/kg5d组和环磷酰胺100mg/kg隔天给药组对肝肾功能的影响更为明显。提示腹腔注射环磷酰胺可建立CD3+T,CD3+CD4+T及CD3+CD8+T细胞明显下降的免疫抑制模型,其中以环磷酰胺50mg/kg5d和80mg/kg3d方式对肝肾功能的损伤较小。BACKGROUND： The immunosuppressive models are commonly used in the research of immunostimulants, but how to establish the immunosuppressive models is the key for the evaluation of immunostimulants. OBJECTIVE： To establish C57BL/6J mice immunosuppressive model induced by cyclophosphamide METHODS： Normal C57BL/6J mice were randomly divided into six groups （n=4）. Group 1 was normal control group, group 2 was injected with 50 mg/kg cyclophosphamide for 5 days, group 3 was injected with 80 mg/kg cyclophosphamide for 3 days, group 4 was injected with 80 mg/kg cyclophosphamide for 5 days, group 5 was injected with 100 mg/kg cyclophosphamide for 5 days, and group 6 was injected with 100 mg/kg cyclophosphamide every 2 days. Mice in group 1 were intraperitoneal injected with 0.1 mL normal saline and lasted for 5 days. Mice in group 2, 3, 4 5 were intraperitoneal injected with 50, 80, 80 and 100 mg/kg cyclophosphamide, respectively, and lasted for 5, 3, 5 and 5 days, respectively. Mice in group 6 were intraperitoneal injected with 100 mg/kg cyclophosphamide every two days and total injected for three times. RESULTS AND CONCLUSION： Compared with group 1, the CD3＋T, CD3＋CD4＋T and CD3＋CD8＋T levels in the peripheral blood in the other five groups were significantly decreased （P 〈 0.05）; the contents of alanine aminotransferase （except for group 2 and group 3）, aspartate aminotransferase and blood urea nitrogen in the other five groups were significantly increased （P 〈 0.05）, especially in group 4, 5 and 6. Intraperitoneal injection of cyclophosphamide can establish the immunosuppressive model with decreased level of CD3＋T, CD3＋CD4＋T and CD3＋CD8＋T, and intraperitoneal injection of 50 mg/kg and 80 mg/kg cyclophosphamide for 5 and 3 days has less kidney and liver damage.

关 键 词：免疫抑制模型 C57BL/6J小鼠 CD3+T淋巴细胞 CD3+CD4+T淋巴细胞 CD3+CD8+T淋巴细胞 环磷酰胺 

分 类 号：R318[医药卫生—生物医学工程]