紧密连接蛋白Occludin在早孕绒毛组织中的表达及其意义  

Expression of tight junction protein Occludin in chorionic villus and its significance

作　　者：冯亚玲[1,2,3] 齐观南[3] 周昌菊[1] 

机构地区：[1]中南大学湘雅三医院妇产科,湖南省长沙市410013 [2]深圳市宝安区人民医院妇产科,广东省深圳市518101 [3]中山大学附属第三人民医院,广东省广州市510630

出　　处：《中国组织工程研究》2012年第40期7528-7532,共5页Chinese Journal of Tissue Engineering Research

基　　金：湖南省科技厅基金资助(2010SK3114);中南大学博士生学位论文创新资助项目(2009bsxt043)~~

摘　　要：背景:Occludin蛋白是否在人早孕期胚泡植入中发挥作用,目前尚不能确定。目的:探讨紧密连接蛋白Occludin在早孕绒毛组织中的表达及意义。方法:早孕的正常人流绒毛组织和稽留流产绒毛组织各30例,用半定量RT-PCR,Western blot和免疫组化表达等各方法验证紧密连接蛋白Occludin在各组绒毛组织中的表达。结果与结论:紧密连接蛋白Occludin主要表达在于绒毛外的合体滋养细胞的胞浆,胞核和少许间质,细胞滋养细胞Occludin表达低。半定量RT-PCR,Western blot和免疫组化表达等各方法验证紧密连接蛋白Occludin在正常人流和稽留流产绒毛组织中均有表达,前者的表达低于后者,但差异无显著性意义(P>0.05)。说明紧密连接蛋白Occludin在绒毛组织有表达,细胞滋养细胞Occludin低表达可能是细胞滋养细胞极性消失的原因之一,参与细胞滋养细胞浸润能力形成,但胚胎发育停滞并不单是细胞滋养细胞侵润能力不足引起。BACKGROUND： The role of Occludin protein in the embryo implantation during early pregnancy has never been reported. OBJECTIVE： To investigate the expression of Occludin in the chorionic villus and to explore its significance METHODS： The mRNA and protein expression of Occludin in chorionic villus from 30 normal women of early pregnancy and 30 missed abortion patients were measured by semi-quantitative reverse transcription-PCR, Western blot and immunohistochemistry methods. RESULTS AND CONCLUSION： Occludin was showed in endochylema, cell nucleus and little stroma of syneytiotrophoblast cell of external chorionic villus and showed low expression in the cytotrophoblast. Semi-quantitative reverse transcription-PCR, Western blot and immunohistochemistry staining found that the Occludin expressed in chorionic villus in normal early pregnancy women and missed abortion patients, the expression in normal early pregnancy was lower than that in missed abortion patients, but there was no significant difference （P 〉 0.05）. There was expression of Occludin in the chorionic villus. The lower expression of Occludin in cytotrophoblast might be one of the reasons for the polarity disappearance of cytotrophoblast cell and participated in the formation of infiltrated capacity of cytotrophoblast. Inadequate cytotrophoblast invasion ability was not the only reason for the embryo development stagnation.

关 键 词：绒毛组织 OCCLUDIN 免疫组化 RT-PCR 免疫印迹 紧密连接蛋白 

分 类 号：R318[医药卫生—生物医学工程]