电针不同经穴对束缚应激模型大鼠行为学影响的实验观察  

Effects of electro-acupuncture at different acupoints on restraint stress-induced behavioral changes in rats

作　　者：孙洁[1] 曾霈君[1] 魏惠芳[1] 温进[2] 仲大奎 李滢[1] 杨磊[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京医学博士研究生100029 [2]北京门头沟区中医医院针灸科,北京102300 [3]北京市房山区中医院康复科,北京102400

出　　处：《医学研究生学报》2012年第10期1027-1031,共5页Journal of Medical Postgraduates

基　　金：北京市自然科学基金(7052037)

摘　　要：目的目前紧张的工作状态和激烈的社会竞争压力等应激源日益泛化,由此导致的身心疾病已严重影响人的生存质量。临床观察电针对应激性精神心理疾病具有明显疗效。文中旨在通过观察电针不同穴位对束缚应激模型大鼠行为改变的影响,进一步探讨免疫失调状态下心理行为的改变及电针的调节作用。方法将50只雄性Wistar大鼠随机分为正常组、模型组、电针百会组、电针关元组、电针足三里组,每组10只。除正常组外,余各组均采用自制布袋束缚大鼠30 min,每天1次,共20 d制备束缚应激大鼠模型,于造模第2天,各治疗组捆绑固定后给予电针20 min,隔天1次,共10次。于造模前、造模后、治疗5次及10次,分别采用高架十字迷宫(elevated plus-maze,EPM)检测各组大鼠进入开放臂次数(open arm entry,OE)比例及停留开放臂时间(open arm time,OT)比例的变化。结果与造模前比较,造模后电针百会组大鼠OT比例降低明显、电针关元组大鼠OE比例升高明显,电针足三里组大鼠造模后、治疗5次后OT比例均明显降低,治疗10次后大鼠OE比例则明显升高,P<0.01;与造模刚结束比较,治疗5次后电针百会组大鼠OT比例明显升高(P<0.05),电针关元组大鼠OE比例明显降低、电针足三里组治疗10次后模型大鼠OE和OT比例均升高明显(P<0.01);与治疗5次比较,治疗10次后电针关元组大鼠OE比例升高明显(P<0.05),电针足三里组大鼠OE和OT比例均明显升高(P<0.01)。电针各组间比较,大鼠在治疗5次、10次后OE和OT比例呈不同程度的升高或降低趋势,差异无统计学意义(P>0.05)。结论电针介入具有一定抗抑郁和抗焦虑作用,可改善慢性束缚应激所引起的抑郁、情绪焦虑;百会、关元、足三里穴均具有抗焦虑作用,且不同穴位效应不同,百会在应激早期的抗焦虑作用明显,而关元、足三里穴的作用较为稳定、持久。Objective Clinical observations show that electro-acupuncture has significant effects on stress-induced psycho- logical disorders. This study aims to investigate the effects of electro-acupuncture at different acupoints on restraint stress-induced be- havioral changes, and discuss the psychological and behavioral changes caused by immunity disorders and the regulatory effect of elec- tro-acupuncture for these problems. Methods Fifty male Wistar rats were randomly divided into 5 groups （ normal, model, Baihui, Guanyuan and Zusanli） of equal number. Apart from those of the normal group, all the other rats were tied into cloth bags for 30 min a day for 20 d to make the restraint stress model. From the 2nd day after modeling, the rats of treatment groups underwent electro-acu- puncture for 20 min, once every other day, for 10 times. All the rats were placed in the elevated plus-maze （EPM） for detection of the changes in the number of entries into the open arms （OE） and residence time in the open arms （OT） of EPM before and after model- ing, and after the 5th and 10th treatment. Results Compared with the normal rats, the rats of the Baihui group showed significantlydecreased OT, while those of the Zusanli group markedly increased number of OEs after the 10th treatment and reduced OT after the 5th （P 〈0. O1 ）. In comparison with those of the model group, the num- ber of OEs was significantly increased in the Baihui group （ P 〈 0.05 ） but decreased in the Guanyuan group after the 5th treatment, and both OT and OEs remarkably increased in the Zusanli group after the 10th treatment （P 〈 O. O1 ）. The number of OEs was significantly higher in the Guanyuan group after the 10th treatment （P 〈 0.05 ）, and so wereOT and OEs in the Zusanli group （P 〈0.01 ） than after the 5th. Compared with the Baihui group, both the Guanyuan and Zusanli groups exhibited changes in OEs and OT, but with no significant differences （P 〉 0.05）. Conclusion Electro-acupuncture has some antidepressant

关 键 词：束缚应激 电针 高架十字迷宫 行为学 

分 类 号：R245.97[医药卫生—针灸推拿学]