心脉隆胶囊对原代大鼠血管平滑肌细胞形态损伤的保护作用  

Protective Effect of Xinmailong Capsules on Morphologic Injury in Primary Cultured Rat Vascular Smooth Muscle Cells

作　　者：肖芬[1] 吴建新[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000

出　　处：《大理学院学报（综合版）》2012年第9期29-32,共4页Journal of Dali University

基　　金：云南省腾冲制药厂开发研究项目(2010TY0910)

摘　　要：目的:探讨大鼠主动脉血管平滑肌细胞(VSMCs)的原代培养方法及观察心脉隆胶囊(XMLJ)对由H2O2诱导VSMCs细胞形态损伤的保护作用。方法:采用组织贴块法培养原代大鼠VSMCs,继而用62.5μmol.L-1H2O2损伤细胞,通过HE染色观察XMLJ对VSMCs形态损伤的保护作用。结果:200μg.mL-1的XMLJ对过氧应激造成的VSMCs形态损伤,具有明显的保护作用,细胞形态接近于正常组VSMCs。结论:组织贴块法培养大鼠VSMCs方便简单,XMLJ对H2O2诱导的VSMCs细胞形态损伤具有一定的保护作用。Objective： To investigate the primary culture method of rat aorta VSMCs and observe the protection of XMLJ on morphologic injury of VSMCs indcued by H 2 O 2.Methods： Rat aorta VSMCs were cultured following tissue-piece inoculation method. Cultured VSMCs were then stimulated with 62.5 μmol·L-1 H 2 O 2 under the condition of with or without XMLJ,HE dyeing method was applied to observe the morphologic changes of VSMCs.Results： With XMLJ 200 μg·mL-1,VSMCs under oxidative stress exhibited nearly no difference compared with the normal cells.XMLJ with 200 μg·mL-1 on VSMCs morphologic change induced by oxidative stress showed obvious protective effect.Conclusion： Tissue-piece inoculation culture of VSMCs is uncomplicated.There was a protective effect of XMLJ on morphologic injury of VSMCs.

关 键 词：大鼠 血管平滑肌细胞 组织贴块法 氧化应激 细胞形态 

分 类 号：R285.5[医药卫生—中药学]