基于Au纳米通道膜分离大豆异黄酮单体的研究  

Separation of soy bean isoflavones based on gold nanotubule membrane

作　　者：樊定艳[1] 彭斌[1] 李平[1] 冯丽萍[1] 黄杉生[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2012年第5期496-501,共6页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家科技部863计划项目(2007AA06Z402);上海市科委项目(08520510400);上海师范大学重点学科(DZL706)

摘　　要：以聚碳酸酯滤膜为模板,采用化学沉积法将Au沉积到膜孔道内,制备了直径约10 nm的Au纳米通道膜(Au-Mem),对巯基苯胺(PATP)通过金-硫键共价自组装至Au纳米通道孔壁上,形成致密单层氨基末端的Au纳米通道膜(PATP-Au-Mem),pATP末端氨基在较低pH值条件下易被质子化而使Au纳米通道膜带正电荷,对于荷正电的物质通过纳米通道膜具有静电斥力作用.利用染料木素与铝可形成荷正电的螯合物后不易通过Au纳米通道的特性,在pH=4.5的条件下,基于PATP-Mem膜分离了大豆异黄酮单体染料木素与大豆黄素,其分离度可达6.91.An electroless plating method was applied to deposit Au onto the walls of pores of polycarbonate membranes （PC- Mem）. p-aminothiophenol was modified onto the walls of the PC-Mem by Self-assembly（PATP-Au-Mem）. The nanotubules were positively charged at lower pH because the amino of the PATP can be easily protonated, and the compound with the positive charge with nanotubules was excluded to transport through the membrane due to the electrostatic repulsion. Genistein reacts with A13 ~ to form a metal chelate with positively charge and is hard to pass through the PATP-Au-Mem, while Daidzein can transport easily through this membrane. In the pH 4.5 medium, Genistein and Daidzein in soy isoflavone were successfully separated with a separate factor of 6.91 based on the PATP-Mem,

关 键 词：Au纳米通道 化学沉积 分离 染料木素 大豆黄素 

分 类 号：O658.9[理学—分析化学]