葡萄球菌中诱导型克林霉素耐药株的检测及分析  

Testing and analysis of induced elindamycin drug resistance bacilli strain among Staphylococcus

作　　者：钟红 

机构地区：[1]湖南沅陵县南方医院检验科,湖南沅陵419600

出　　处：《医学检验与临床》2012年第5期58-60,共3页Medical Laboratory Science and Clinics

摘　　要：目的用克林霉素诱导耐药实验（D实验）调查湖南沅陵县南方医院葡萄球菌中诱导型克林霉素耐药菌株的发生率，以期为临床合理应用抗菌药物提供依据。方法应用TDR半自动微生物分析系统及VITEK一2全自动微生物分析系统对临床分离葡萄球菌进行鉴定及药敏分析，按照美国临床实验室标准化委员会（CLSL／NCCLS2011版）标准进行D试验。结果临床收集的104株对红霉素耐药而对克林霉素敏感葡萄球菌标本中有55株（52．88％）产生诱导型耐药，其中在MRSA中为77．78％，高于MssA（61．90％）和CONS（22．85％）。结论临床标本中分离出的对红霉素耐药而对克林霉素敏感的葡萄球菌在进行常规药敏实验的同时，应开展D实验检测葡萄球菌对克林霉素的耐药性，可帮助临床医生正确的选用大环内酯类、林克酰胺类、链阳霉素B类抗生素。Objective To investigate the infection of clindamycin- induced resistance among Staphylococcus by induction test （D test） and to provide guidance for the proper use of antibiotics. Methods CLinical isolates were identified by TDR and VITEK - 2. The inducible resistance to clindamycin was checked by D - test according to The Standards of CLSL/NCCLS2010. Results Totally 104 strains of erythromycin re-istant and clindamycin- sensitive staphylococcus collected from various samples, there are 55 straius of staphylococcus was clindamycin - induced resistance which appeared resistant to erythromycin and sensitive to elindamycin , then the positive of D test take up 52.88 %, In which MRSA was 77.78%, higher than the the MSSA （61.90%） and the CONS （22.85%）. Conclusion The inducible resistance of eryth.romycin to clindamycin in Staphylococcus should be checked by D - test in clinical laboratory in order to help physicians to select marolides antibiotics correctly.

关 键 词：红霉素 克林霉素 D实验 诱导型耐药 葡萄球菌 

分 类 号：R978.1[医药卫生—药品]