高胆固醇饮食豚鼠胆囊组织中c-kit和scf mRNA及蛋白的表达变化研究  

Changes of c-kit and scf mRNA and Protein in Guinea Pig Gallbladder Fed on High Cholesterol Diet

作　　者：范莹[1] 吴硕东[1] 付倍蓓[2] 殷振华[1] 

机构地区：[1]中国医科大学附属盛京医院普外二科,辽宁沈阳110004 [2]中国医科大学附属盛京医院儿科,辽宁沈阳110004

出　　处：《中国普外基础与临床杂志》2012年第10期1063-1068,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(项目编号:81000183)~~

摘　　要：目的探讨豚鼠胆囊胆固醇结石形成过程中c-kit及scf mRNA及蛋白的表达变化情况。方法将20只豚鼠随机分为2组,分别给予普通饲料及致石饲料,致石周期为6周。RT-PCR检测胆囊组织中c-kit及scfmRNA的表达情况,Western blot检测c-kit及scf蛋白的表达情况。结果 RT-PCR结果显示,与对照组相比,致石组豚鼠胆囊c-kit mRNA(t=6.985,P<0.01)和scf mRNA(t=6.028,P<0.01)的表达量下降。Western blot结果显示,与对照组相比,致石组豚鼠胆囊c-kit(t=10.256,P<0.01)及scf蛋白(t=9.586,P<0.01)表达量下降。结论饮食诱导的豚鼠胆囊胆固醇结石形成过程中,豚鼠胆囊c-kit和scf mRNA及蛋白表达水平均下降。c-kit/scf通路抑制可能参与胆囊胆固醇结石的形成。Objective To discuss the changes of c-kit/scf mRNA and protein in guinea pig gallbladder fed on high cholesterol diet.Methods Twenty guinea pigs were divided into two equal groups of 10 each：the control group and lithogenic group.Normal diet and high cholesterol diet was given to each group respectively.The period of stone permeation was six weeks.RT-PCR and Western blot were used to determin the expressions of c-kit and scf mRNA and protein.Results RT-PCR results showed that the expressions of c-kit mRNA（t=6.985,P0.01） and scf mRNA（t= 6.028,P0.01）decreased significantly in lithogenic group compared with the control group.Western blot results showed that the expressions of c-kit protein（t=10.256,P0.01） and scf protein（t=9.586,P0.01）decreased significantly in lithogenic group compared with the control group.Conclusions The expressions of c-kit/scf mRNA and protein decrease during the formation of cholesterol gallstones in guinea pigs fed on high cholesterol diet.Inhibition of c-kit/scf pathway may play a role in the formation of cholesterol gallstones.

关 键 词：胆固醇结石 C-KIT基因 干细胞因子 逆转录聚合酶链反应 蛋白印迹 

分 类 号：R657.4[医药卫生—外科学]