采用ISSR-PCR分子标记法鉴别黑果枸杞与雄性不育枸杞  

Identification of Lycium ruthenicum Murr and Male Sterility of Lycium barbarum L.by Inter-Simple Sequence Repeat Markers

作　　者：思彬彬[1] 张靠稳[1] 刘国迪[1] 

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《安徽农业科学》2012年第33期16094-16095,共2页Journal of Anhui Agricultural Sciences

基　　金：北方民族大学资助项目(2010Y045)

摘　　要：[目的]探索用ISSR分子标记方法在核酸分子水平上鉴别黑果枸杞与雄性不育枸杞。[方法]从100条ISSR引物中筛选合适的引物对黑果枸杞与雄性不育枸杞样品进行PCR扩增及电泳分析,寻找特征位点。[结果]有4条ISSR引物扩增出较为明显的多态性特征条带,可单独应用于黑果枸杞与雄性不育枸杞的鉴别。[结论]ISSR作为一种简便、可靠的分子标记方法,可用于黑果枸杞与雄性不育枸杞的鉴别。[Objective] To identify Lycium ruthenicum and male sterility of Lycium barbarum by inter-simple sequence repeat markers.[Method] Primers suitable for routine analysis were screened from 100 inter-simple sequence repeat primers,then,they were used in PCR and separated of 2 samples of L.ruthenicum and male sterility of L.barbarum L.[Results] Four of the 100 primers amplified polymorphic bands and suitable for the identification of L.ruthenicum and male sterility of L.barbarum.[Conclusion] Inter-simple sequence repeat markers provide a quick,reliable molecular marker for identification of L.ruthenicum and male sterility of L.barbarum.

关 键 词：枸杞 ISSR 鉴别 

分 类 号：S567.19[农业科学—中草药栽培]