枳早期结瘤素样基因PtBCP1的克隆和分析  

Clone and Analysis of A Phytocyanin-related Early Nodulin-like gene,PtBCP1 from Poncirus trifoliate

作　　者：姚利晓[1,2] 马园园 李凤龙[1,2] 许兰珍 雷天刚[1,2] 彭爱红 何永睿[1,2] 陈善春 

机构地区：[1]中国农业科学院 [2]西南大学柑桔研究所国家柑桔品种改良中心国家柑桔工程技术研究中心,重庆400712

出　　处：《安徽农业科学》2012年第33期16102-16106,共5页Journal of Anhui Agricultural Sciences

基　　金：长江学者和创新团队发展计划项目(PCSIRT);柑桔产业技术体系(CARS-27)农业部公益性行业(农业)科研专项(2010 03067-02-4);重庆市自然科学基金计划项目(cstc2011jjA811)

摘　　要：[目的]克隆枳早期结瘤素样基因PtBCP1并对其序列进行分析。[方法]以枳消减文库中的C28 EST为种子序列进行电子克隆,据此设计引物进行PCR,以获得枳早期结瘤素样基因PtBCP1的全长cDNA和DNA序列,并对获得的序列进行生物信息学分析。[结果]PtBCP1基因由2个外显子和1个内含子组成,在枳幼苗根中的表达量是叶中的140倍,该基因编码的蛋白含131个氨基酸残基,预测分子量为14.0 kD,理论等电点为8.75,具有N端信号肽和PCLD(PFMD:PF02298)功能域,但无完整的铜离子结合位点,属于早期结瘤素样蛋白。[结论]为进一步研究PtBCP1基因的生物学功能奠定了基础。[Objective] The aim was to clone and analyze the sequence of phytocyanin-related early nodulin-like gene（PtBCP1） from Poncirus trifoliata.[Method] The electronic cloning was done by using the C28 EST of Poncirus trifoliata subtractive library as seed sequences and the RT-PCR primers were designed by that.Then the PtBCP1 cDNA and DNA of Poncirus trifoliate were cloned by PCR and the sequences were analyzed by bioinformatics methods.[Result] The PtBCP1 gene included two exons and one intron,and was 140 times of expression in the seeding root of Poncirus trifoliata than that of in leaves.The gene encoded a polypeptide of 131 amino acids with a 14 kD molecular weight and 8.75 isoelectric point.The PtBCP1 protein was a early nodulin-like protein,with a signal peptide in the N-terminal and a plastocyanin-like domain in the C-terminal,and without the complete copper-binding sites.[Conclusion] It prepared the ground for the further research of PtBCP1 gene biological function.

关 键 词：枳 早期结瘤素样基因 克隆 

分 类 号：S188[农业科学—农业基础科学]