云芝漆酶对靛蓝废水脱色降解研究  

Decolorization on Indigo Dyeing Wastewater by Laccase from Coriolus versicolor

作　　者：高恩丽[1,2] 仲伟佳[3] 付小兰[1,2] 陈福山[1,2] 叶志刚[1,2] 

机构地区：[1]九江学院化学与环境工程学院,江西九江332005 [2]九江学院江西省鄱阳湖生态经济研究中心,江西九江332005 [3]九江学院继续教育学院,江西九江332005

出　　处：《安徽农业科学》2012年第33期16321-16324,共4页Journal of Anhui Agricultural Sciences

基　　金：江西省教育厅青年基金项目(GJJ09596);国家自然基金项目(51064011)

摘　　要：[目的]探讨云芝漆酶对靛蓝废水的脱色净化作用。[方法]首先研究不同温度、pH、靛蓝浓度、HBT添加量、漆酶用量对漆酶/HBT脱色作用的影响,然后分析漆酶和酸性纤维素的酶协同水洗。[结果]当以1-羟基苯并三唑(简称HBT)为介体时,云芝漆酶/HBT对靛蓝作用的动力学参数Km和Vmax分别为0.318 mmol/L和0.035 5 mmol/(L.min)。在温度50℃,pH 4.5,靛蓝浓度100 mg/L,介体HBT用量0.1%,漆酶用量100 IU/L条件下,作用40 min时,靛蓝脱色率高达96.5%。牛仔布生物整理中采用漆酶与酸性纤维素酶协同作用,当漆酶用量为15 000 IU/kg牛仔布时,牛仔布的返沾程度减少85%,同时水洗废水中靛蓝含量减少83.8%。[结论]漆酶在牛仔服的生物整理及染整废水的脱色净化处理中具有良好的应用前景。[Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor.[Method] Firstly,the effects of temperature,pH,indigo concentration,HBT concentration,laccase dosage on the decolorization of indigo dyeing wastewater by laccase/HBT,and then the synergism of laccase and acid cellulase was analyzed.[Result] Using ABTS as the substrate,the kinetic parameters,Km and Vmax,were 0.318 mmol/L and 0.035 5 mmol/（L·min） respectively.The decolorization rate of indigo reached 96.5% with HBT as introducer for 40 min at temperature 50 °C,pH=4.5,indigo concentration 100 mg/L,HBT concentration 0.1% and laccase dosage 100 IU/L.Reducing backstaining of blue jeans by the synergism of laccase and acid cellulase during the bio-finishing was experimented,and the results showed that the backstaining degree of blue jeans was reduced by 85% when the amount of laccase was 15 000 IU/kg.Besides,the synergism of laccase and acid cellulase decreased the amount of indigo in wastewater by 83.8%.[Conclusion] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater.

关 键 词：漆酶 云芝菌 靛蓝 脱色 染整废水 

分 类 号：S188[农业科学—农业基础科学]