Telencephalin protects PAJU cells from amyloid beta protein-induced apoptosis by activating the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway  

作　　者：Heping Yang Dapeng Wu Xiaojie Zhang Xiang Wang Yi Peng Zhiping Hu 

机构地区：[1]Department of Neurology,Shangrao No.5 People's Hospital,the Second Affiliated Hospital of Nanchang University,Shangrao 334000,Jiangxi Province,China [2]Department of Neurology,Ruikang Hospital Affiliated to Guangxi Traditional Chinese Medical University,Nanning 530011,Guangxi Zhuang Autonomous Region,China [3]Department of Neurology,Xiangya Second Hospital,Central South University,Changsha 410011,Hunan Province,China [4]Department of Psychiatry,Xiangya Second Hospital,Central South University,Changsha 410011,Hunan Province,China

出　　处：《Neural Regeneration Research》2012年第28期2189-2198,共10页中国神经再生研究（英文版）

基　　金：supported by a grant under Key Projects of Guangxi Traditional Chinese Medical University, No.ZD2007041

摘　　要：Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid, while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin activates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis.Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid, while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin activates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis.

关 键 词：telencephalin/intercellular adhesion molecule 5 amyloid beta protein ezrin/radixin/moesin familyproteins/phosphatidylinositol-3-kinase/protein kinase B signal transduction neural regeneration 

分 类 号：Q421[生物学—神经生物学] Q556.3[生物学—生理学]