高血压患者血管紧张素Ⅱ受体基因多态性与颈动脉硬化间的关系  

The Relationship between Angiotensin Ⅱ Type 1 Receptor Gene Polymorphism and Carotid Artery Arteriosclerosis in Essential Hypertension Patients

作　　者：林从容 吴可贵 谢良地 柯晓刚[2] 叶琼 

机构地区：[1]福建省高血压研究所 [2]福建医科大学附属第一医院B超室,福建省福州市350005

出　　处：《中国动脉硬化杂志》2000年第2期157-160,共4页Chinese Journal of Arteriosclerosis

基　　金：福建省科委基金资助项目!(项目编号98A061)

摘　　要：为探讨血管紧张素Ⅱ 1型受体基因多态性与原发性高血压及与高血压颈动脉硬化之间的关系 ,将高血压患者 (n =12 0 )和正常对照者 (n =86 )进行血压、身高、体重、空腹血糖及血清总胆固醇和甘油三酯浓度测定 ,用饱和盐析法常规提取外周血白细胞DNA ,采用多聚酶链式反应结合限制性内切酶法检测血管紧张素Ⅱ 1型受体基因的C116 6等位基因在正常人和原发性高血压患者中的频率。并测定正常对照组 (n =32 )和高血压组 (n =6 8)颈总动脉内膜 -中膜厚度、管腔内径及壁 腔比值。结果发现 ,高血压患者血管紧张素Ⅱ 1型受体基因AC基因型频率比正常对照者高 (P <0 .0 1) ;高血压组C116 6等位基因频率高于正常对照组 (P <0 .0 5 ) ;AC基因型高血压患者颈总动脉内膜 -中膜厚度比AA基因型高血压患者厚 (P <0 .0 5 ) ,AC基因型高血压患者壁 腔比值大于AA基因型高血压患者 (P <0 .0 1) ,AA和AC基因型高血压患者颈总动脉内径无差别。提示血管紧张素Ⅱ 1型受体基因A116 6C多态性与中国人高血压病有关 。Aim To investigate the relationships between polymorphism of candidate gene and carotid artery arteriosclerosis in essential hypertension (EH) patients, the frequencies of C1166 allele of angiotensin II type 1 receptor (AT 1R) gene was determined in normotensives and hypertensives Methods Blood pressure (Bp), body mass index (BMI), fasting serum glucose (Glu), serum total cholesterol (TC) and triglyceride (TG) were measured in 86 normotensives and 120 hypertensives. Intimal-medial thickness (IMT), diameter (D) and I/D of common carotid artery in 32 normotensives and 68 hypertensives were determined. Genomic DNA was extracted from peripheral blood leukocytes by standard method. Polymerase chain reaction (PCR) combined with restriction enzyme digestion was used to detect the polymorphism. Results The frequency of AC genotype and C1166 allele of AT 1R gene of EH patients were higher than that of control (P<0.01 and P<0.05), EH subjects with AC genotype had increased common carotid artery IMT and I/D than those with AA genotype (P<0.05 and P<0.01). Conclusions A1166C polymorphism of AT 1R gene were associated with EH in Chinese population. C1166 allele of AT 1R gene may play a role in arteriosclerosis of hypertension.

关 键 词：原发性高血压 血管紧张素Ⅱ 动脉硬化 PCR 

分 类 号：R544.1[医药卫生—心血管疾病]