极低密度脂蛋白受体基因CGG串联重复序列多态性与冠心病的关系  

The Correlation between CGG Triplet Repeat Ploymorphism of Very Low Density Lipoprotein Receptor Gene and Coronary Heart Disease

作　　者：邵华[1] 周新[2] 李艳[1] 胡波[3] 

机构地区：[1]湖北医科大学附属第一医院检验科,湖北省武汉市430060 [2]湖北医科大学附属第二医院检验科,湖北省武汉市430071 [3]中山医科大学附属第三医院检验科,广东省广州市510120

出　　处：《中国动脉硬化杂志》2000年第2期169-171,共3页Chinese Journal of Arteriosclerosis

摘　　要：极低密度脂蛋白受体基因 5’非编码区起始密码子上游 19bp处存在CGG三核苷酸串联重复序列 ,为探讨中国汉族人群极低密度脂蛋白受体基因CGG串联重复序列多态性与冠心病的相关性 ,采用聚合酶链反应、变性聚丙烯酰胺凝胶电泳和银染等技术分析湖北地区 75例冠心病患者和 16 0例正常汉族人CGG串联重复序列。共检出CGG串联重复次数为 5、8、9、11的四种等位基因及 5 5、5 8、8 8、8 9、9 9、5 9、8 11和 5 11等八种基因型 ,冠心病组和对照组极低密度脂蛋白受体等位基因频率和基因型频率分布无统计学差异 (P >0 .0 5 ) ,结果提示 。Aim To explore the correlation between CGG triplet repeat polymorphism on the 5' non-coding region of very low density lipoprotein receptor (VLDLR) gene and coronary heart disease (CHD) in Chinese Han nationality, 235 subjects with no blood links in Hubei region, including 75 cases with CHD and 160 healthy controls were studied. Methods Polymerase chain reaction (PCR)-denature polyacrylamide gel electrophoresis-silver stain technique was employed to determine the CGG triplet repeat polymorphism of VLDLR gene. Results The Chinese Han nationality in Hubei region has VLDLR gene polymorphism, 4 alleles with 5, 8, 9, 11 CGG triplet repeats and 8 genotypes with 5/5, 5/8, 8/8, 8/9, 9/9, 5/9, 8/11 and 5/11 were detected. There were no statistically significant differences in VLDLR gene allele and genotype frequencies between CHD and normal controls (P>0.05). Conclusions There is VLDLR gene polymorphism in the Han Chinese in Hubei region and such polymorphism has ethnic difference. CGG triplet repeat polymorphism of VLDLR gene has no correlation with CHD. It is not an independent risk factor

关 键 词：极低密度脂蛋白 基因 多态性 冠心病 PCR 

分 类 号：R541.4[医药卫生—心血管疾病]