重组腺相关病毒介导的IL-32基因抑制人瘢痕疙瘩成纤维细胞增殖的机制研究  

Study on recombinant adeno-associated virus-mediated IL-32 gene inhibits Human keloid fibroblast proliferation mechanism

作　　者：张培华[1] 李小川[1] 李瑾[1] 邹雅琴[1] 梁杰[1] 

机构地区：[1]广东医学院整形外科研究所,广东湛江524001

出　　处：《中国美容医学》2012年第11期1987-1990,共4页Chinese Journal of Aesthetic Medicine

基　　金：广东省自然科学基金项目(9151008002000010)

摘　　要：目的:探讨重组腺相关病毒介导的IL-32基因抑制人瘢痕疙瘩成纤维细胞(Keloid fibroblasts,KFs)增殖的机制。方法:用重组腺相关病毒介导的IL-32(rAAV2IL-32)及腺相关病毒空载体感染人KFs,通过荧光显微镜观察rAAV2IL-32的感染效率,RT-PCR法检测IL-32的转录水平;用MTT法检测KFs增殖的情况;用蛋白质印迹方法检测Bcl-2、BAX、TGF-β1的表达。结果:rAAV2IL-32及腺相关病毒空载体感染KFs 48h后,RT-PCR检测显示rAAV2IL-32组出现高表达电泳条带,而腺相关病毒空载体组则未出现电泳条带;MTT法检测显示r AAV2I L-32组明显抑制了KFs的增殖;蛋白质印迹结果显示rAAV2IL-32组KFs中Bcl-2、TGF-β1表达降低,BAX表达增高。结论:rAAV2IL-32可能是通过减少KFs中Bcl-2和TGF-β1的表达以及增加BAX的表达来抑制瘢痕疙瘩的增殖。Objective Explore the mechanism of IL-32 gene regulation in Keloid fibroblasts （KFs）. Methods With adeno-associated virus-mediated IL-32 （rAAV2 IL-32） and empty vector of adeno-associated virus infection of human KFs. Infection efficiency of adeno-associated virus was observed by fluorescence microscopy.Transcription levels of IL- 32 was detected by RT-PCR assay.The effects of IL-32 on KFs cell proliferation was detected by MTT.BcI,BAX,TGF-β1 expression was detected by Western blot. Results After of rAAV2 IL-32 and adeno-associated virus empty vector infection KFs 48h,RT-PCR test results showed rAAV2 IL-32 group of high expression of electrophoretic bands,adeno- associated virus vector group did not appear electrophoretic bands.MTT assay showed that of rAAV2 IL-32 significantly inhibited the KFs proliferation.Western blot results showed that overexpression of Bct IL-32 KFs,reduced expression of TGF-β1,increased expression of BAX. Conclusion IL-32 to inhibit proliferation of scar may reduce the expression of Bcl and TGF-β1and increased expression of BAX.

关 键 词：白细胞介素32 瘢痕疙瘩 Bcl-2 TGF-β1 BAX 

分 类 号：R619.6[医药卫生—外科学]