IL-32在病理性瘢痕中的表达及其生物学作用研究  

Study on the expression of IL-32 in pathological scars and biological effect in cicatrization

作　　者：李小川[1] 张培华[1] 李瑾[1] 邹雅琴[1] 梁杰[1] 

机构地区：[1]广东医学院整形外科研究所,广东湛江524001

出　　处：《中国美容医学》2012年第11期1991-1993,共3页Chinese Journal of Aesthetic Medicine

基　　金：广东省自然科学基金项目(9151008002000010)

摘　　要：目的:探讨IL-32在增生性瘢痕和瘢痕疙瘩的形成过程中所起的生物学作用。方法:收集2009年10月至2011年6月广东医学院附属医院整形外科手术切除的瘢痕疙瘩组织12例,增生性瘢痕组织12例,正常皮肤24例,分别应用免疫组织化学技术、RT-PCR和Western Blot检测IL-32在它们中的表达情况。结果:IL-32在正常皮肤增生性瘢痕和瘢痕疙瘩组织中均有表达,在正常皮肤中表达较强,在增生性瘢痕及瘢痕疙瘩组织中表达较弱;IL-32mRNA和IL-32蛋白在增生性瘢痕和瘢痕疙瘩中的表达较正常皮肤明显降低(P均<0.05),而增生性瘢痕组和瘢痕疙瘩组之间差异无统计学意义(P>0.05)。结论:IL-32在病理性瘢痕组织的形成过程中起着一定的重要作用,有进一步研究的价值。Objective To explore the biological contribution of IL-32 in the formative course of hypertrophic scar and keloid. Methods From October 2009 to June 2011, 12 cases of keloid tissue; 12 cases of hypertrophic scar tissue; 24 cases of normal skin were collected at Guangdong Medical College Hospital plastic surgical. Take normal skin as control group, the IL-32 mRNA of tissue are investigated in the specimens from normal skin, hypertrophic scar and keloid tissues using reverse transcription polymerase chain reaction （RT-PCR）. Further immunohistochemical technique and western blot are performed to detect the expression and distribution of IL-32 protein in the paraffin embedded tissues samples of normal skin, hypertrophic scar and keloid. Results The expression of IL-32 mRNA was obviously lower in hypertrophic scar and keloid tissues compared with those of normal skin respectively. The result of immunohistochemical technique and Western Blot： the expression of tL-32 protein were both lower in hypertrophic scar and keloid tissues compared with those of normal skin respectively（P〈0.05）. Conclusion The results indicated that IL- 32 played an important role in the proliferation of fibroblasts and in the development of pathological scar, the value of further research.

关 键 词：IL-32 增生性瘢痕 瘢痕疙瘩 

分 类 号：R619.6[医药卫生—外科学]