川续断干预正畸力作用下兔牙周组织中血小板衍生生长因子BB的表达  

Dipsacaceae intervenes the expression of platelet derived growth factor-BB in the periodontal tissue of rabbits during orthodontic tooth movement

作　　者：赵刚[1] 张欢 孙佳宁 蒋丽妍 

机构地区：[1]佳木斯大学口腔医学院正畸科,黑龙江省佳木斯市154000

出　　处：《中国组织工程研究》2012年第37期6885-6888,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:有研究发现一些中草药在骨折愈合过程中可从不同途径加速骨组织的改建,促进骨折愈合。目的:观察中药川续断水煎液对家兔正畸牙移动及牙移动过程中血小板衍生生长因子BB表达的影响。方法:将27只家兔随机分为实验组(n=12)、对照组(n=12)和空白对照组(n=3,不做任何处置)。实验组与对照组建立双侧下颌第一磨牙近中移动正畸加力模型后,实验组每日灌服3g/kg川续断水煎液,对照组每日灌服等量生理盐水,加力后1,7,14,21d在测量牙齿移动距离后行血小板衍生生长因子BB免疫组织化学分析。结果与结论:加力后7,14,21d,实验组牙移动距离大于对照组(P<0.05)。正畸加力后,随天数增加实验组与对照组血小板衍生生长因子BB的表达均呈逐渐增强趋势,实验组加力后7,14,21d血小板衍生生长因子BB水平高于对照组(P<0.05)。提示川续断水煎液可促进家兔正畸牙齿移动,并上调血小板衍生生长因子BB的表达。BACKGROUND：Studies have found that some Chinese herbal medicines in the process of fracture healing can accelerate bone tissue remodeling by different ways to promote fracture healing. OBJECTIVE：To study the influence of Dipsacaceae decoction on the expression of platelet derived growth factor-BB during orthodontic tooth movement. METHODS：Twenty-seven rabbits were randomly divided into experimental group （n=12）, control group （n=12）, and blank control group （n=3）. Mandibular first molars were moved bilaterally in the experimental and control groups. Then, 3 g/kg Dipsacaceae decoction was given orally to the experimental group, and the equal amount of saline was given orally to the control group. There was no treatment in the blank control group. After 1, 7, 14, 21 days, the distance of tooth movement was measured separately and then immunohistochemical analysis was performed for platelet derived growth factor-BB. RESULTS AND CONCLUSION：After 7-, 14-, and 21-day loading, the tooth movement distance was greater in the experimental group than in the control group （P 0.05）. After orthodontic force was performed, the expression of platelet derived growth factor-BB was gradually increased with time in the experimental and control groups. After 7, 14 and 21 days, the experimental group had a higher expression of platelet derived growth factor-BB compared with the control group （P 0.05）. These point out that Dipsacaceae decoction can promote the orthodontic tooth movement in rabbits and increase the expression of platelet derived growth factor-BB.

关 键 词：川续断 正畸牙移动 牙周组织改建 血小板衍生生长因子BB 距离 组织构建 

分 类 号：R318[医药卫生—生物医学工程]