PI3K/Akt信号通路应力与应激对β-连环蛋白及骨代谢的共刺激  

Costimulatory effect of mechanical stress and body stress on beta-catenin and bone metabolism in the phosphoinositide 3 kinase/protein kinase signal pathway

作　　者：鲍捷[1,2] 王国祥[1] 

机构地区：[1]苏州大学体育学院,江苏省苏州市215021 [2]苏州大学捷美生物医学工程仪器联合重点实验室,江苏省苏州市215006

出　　处：《中国组织工程研究》2012年第37期7003-7008,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省2011年度普通高校研究生科研创新计划项目(CXZZ11_0069);2011江苏省省属高校自然科学研究面上项目(11KJD320001)资助~~

摘　　要：背景:运动时应力作用于人体,且同时伴有应激产生,Wnt/β-连环蛋白(β-catenin)信号通路对应力刺激敏感,通过中间途径环氧化酶2/前列腺素E2调节下游促红细胞生成素/RANKL/RANK信号从而影响骨代谢。白细胞介素6是通过非受体型的酪氨酸激酶/信号传递转录激活蛋白信号通路途径,同样影响骨代谢。目的:探讨应力和应激是否在同一信号途径上对β-连环蛋白的共刺激效应。方法:由第一作者于2000/2011通过计算机检索CNKI、HighWire和Elsevier数据库中关于"应力刺激,应激,Wnt/β-连环蛋白,磷脂酰肌醇-3激酶/丝/苏氨酸蛋白激酶,骨代谢"的相关文献。选择与应力刺激或应激对信号通路的影响有关文章内容,共纳入37篇文章。结果与结论:环氧化酶2/前列腺素E2作为对应力敏感的Wnt/β-连环蛋白信号通路的中间信号,协同应激产生的白细胞介素6,通过对PI3K/Akt途径的激活作用,使β-连环蛋白信号级联放大,并对促红细胞生成素/RANKL/RANK骨吸收信号的调节作用进一步加强,可以解释过度训练导致骨损伤易感性发生的机制,同时也验证了应力与应激在信号通路中的协同刺激作用。BACKGROUND：Exercising mechanical stress affect the body with the body stress. Wnt/β-catenin signal pathway is the most classic signal in the Wnt signal family with the mechanical stress sensitivity. It could change the mechanics signal into biochemistry signal, through making the cyclooxy enase-2/prostaglandin E-2 （COX-2/PGE-2） signal as a medium to accommodate downstream osteoprotegerin receptor activator of nuclear factor-κB ligand, receptor activator of nuclear factor κB （OPG/RANKL/RANK） signal and influence bone metabolism. Interleukin-6 is a signal pathway which transfers the message through the Janus kinase/signal transduction and activation of transcription （JAK/STAT） and influences bone metabolism. OBJECTIVE：To explore the costimulatiory effect of mechanical stress and body stress on β-catenin whether is in the same signal pathway. METHODS：An online search of CNKI, HighWire and Elsevier databases was performed for articles related to ＂mechanical stress, body stress, wnt/β-catenin, PI3K/Akt, bone metabolism＂. Articles published from 2000 to 2011 and related to the effects of mechanical stress and body stress on the signaling pathways were selected. A total of 37 articles were included. RESULTS AND CONCLUSION：As a medium of wnt/β-catenin signal way with the mechanical stress sensitivity, COX-2/PGE-2 cooperated with the Interleukin-6 produced by body stress to amplify the β-catenin signal through activating phosphoinositide 3 kinase /protein kinase （PI3K/Akt） signal pathway, and strengthen the regulatory effect of OPG/RANKL/RANK bone receiving signal pathway. These could explain the mechanism of susceptibility bone injury caused by excessive training and verify the costimulatory effect of mechanical stress and body stress on β-catenin in the signal pathway.

关 键 词：应力 应激 PI3K/Akt Β-连环蛋白 环氧化酶2 前列腺素E2 白细胞介素6 

分 类 号：R318[医药卫生—生物医学工程]