高糖环境对体外培养大血管内皮细胞产生TGF-β的影响  

Effect of high glucose condition on the secretion of TGF- β by cultured macrovascular endothelial cells

作　　者：刘小菁[1] 赵铁耘[1] 李秀钧[1] 强欧[1] 

机构地区：[1]华西医科大学附一院内科实验室,成都610041

出　　处：《职业卫生与病伤》2000年第2期70-72,共3页Occupational Health and Damage

摘　　要：目的 :探讨体外高糖环境对大血管内皮细胞产生转化生长因子 -β(TGF-β)的影响。方法 :分别用含不同浓度葡萄糖 (12 .5 m M、2 5 m M、5 0 m M D-葡萄糖 )、高糖加肿瘤坏死因子 -α(TNF-α)和高糖加胰岛素的 10 %小牛血清 RPMI16 40液培养 SD大鼠主动脉血管内皮细胞 48h,取上述培养液分别用生物学方法测其 TGF-β1 水平 ,采用放射免疫法测其内皮素 (Endothelin ET)的水平。结果 :高糖环境下血管内皮细胞分泌的总 TGF-β1 (总 TGF-β1 含量为 40 6± 2 9vs189± 2 3pg/ ml,P<0 .0 5 )及活性 TGF-β1 (活性 TGF-β1 含量为 16 1± 2 4vs 39.5± 8pg/ ml,P<0 .0 5 )明显增加。TNF-α可以加强高糖的这种刺激作用 ,而胰岛素处理可以抑制这种作用。结论Objective This study was designed to examine the secretion of TGFβ by cultured rat macrovascular endothelial cells under high glucose condition.Methods SD rat aortic endothelial cells were isolated and cultured in 10% FBS RPMI1640 media with various high glucose concentration(12.5mM,25mM or 50mM D glucose),high glucose and TNFα or high glucose and insulin for 48 hours,and the supernatant obtained from the cultured aortic endothelial cells were collected.TGFβ1 level in the supernatant was measured using a standard bioassay,which utilizes the TGFβ1 sensitive MvlLu(CLL 64)mink lung epithelial cell line,and the production of endothelin(ET)in the supernatant was assessed by RIA(radio immunoassay).Results Both total TGFβ 1 (406±29 vs 189±23 pg/ml , P <0.05)and active TGFβ 1(161±24 vs 39.5±8 pg/ml)secreted by the cultured aortic endothelial cells under high glucose condition(25mM)were significantly increased as compared with control.TNFα could enhance the effect of high glucose,while insulin showed inhibition to the secretion of TGFβ 1. Conclusion TGFβ 1 may be involved in the development of diabetic macrovascular complication.

关 键 词：高糖 大血管内皮细胞 转化生长因子-Β 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]