荧光定量PCR检测病理组织中结核分枝杆菌的临床价值  

Clinical value of quantitative PCR technology for detection of mycobacterium tuberculosis in pathological tissue

作　　者：余琦[1] 李宁[1] 宋业颖[1] 李彩云[1] 杨江辉[1] 

机构地区：[1]解放军第309医院病理科,北京100091

出　　处：《局解手术学杂志》2012年第6期590-592,共3页Journal of Regional Anatomy and Operative Surgery

摘　　要：目的探讨应用荧光定量PCR(FQ-PCR)技术在病理组织中检测结核分枝杆菌DNA(TB-DNA)的临床应用价值。方法对196例临床诊断结核的穿刺病理活检标本进行石蜡包埋切片,采用实时荧光定量PCR技术检测TB-DNA,并行抗酸染色及组织病理学检查,对三种检测与临床诊断符合率进行比较。结果在196例临床诊断结核的石蜡包埋组织中,荧光定量PCR检测阳性136例,阳性率为69.39%,抗酸染色检测阳性69例,阳性率为35.20%,组织病理学检查阳性102例,阳性率为52.04%,荧光定量PCR检测阳性率最高。结论荧光定量PCR技术简便、快捷,敏感性强、特异性高,可作为结核病分子病理诊断的重要检测方法,具有较高的临床应用价值。Objective To explore the application of fluorescent quantitative PCR（FQ-PCR） and its clinical value for histopathological detection of mycobacterium tuberculosis DNA（TB-DNA）. Methods The paraffin-embedded needle biopsy sections from 196 cases with tu- berculosis were detected by quantitative PCR technique with TB-DNA in real time, and acid-fast staining and histopathological examinations were conducted at the same time. Results Among 196 cases with the clinical diagnosis of tuberculosis in paraffin-embedded tissues, there were 136 cases（69.39% ） screened positive by fluorescence quantitative PCR, 69 cases （35.20%） screened positive by acid-fast staining examination, and 102 cases（52.04% ） screened positive by histopathological examination. The fluorescence quantitative PCR detection is of the highest positive rate. Conclusion The fluorescence quantitative polymerase chain reaction technology is simple, efficient, and of sensitiv- ity and high specificity. Since it is of high clinical value, it can be used as a pathological diagnostic method for tuberculosis molecular detec- tlon

关 键 词：结核分枝杆菌 病理学 PCR 

分 类 号：R446.8[医药卫生—诊断学] R378.91[医药卫生—临床医学]