支配大鼠膀胱的神经在脊髓内的分布—WGA-HRP逆行示踪研究  

The distribution of rat bladder nerves within the spinal cord:WGA-HRP retrograde tracing study

作　　者：胡力丰[1,2] 张志成[1] 孙天胜[1] 

机构地区：[1]北京军区总医院全军创伤骨科研究所,北京100700 [2]第三军医大学,在读研究生重庆400038

出　　处：《临床和实验医学杂志》2012年第22期1768-1769,1771,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的行膀胱逆行示踪,明确支配大鼠膀胱的传入及传出神经在脊髓的位置。方法正常成年SD雄性大鼠(250～300 g),大鼠膀胱壁内注射麦芽凝集素-辣根过氧化物酶(WGA-HRP),注射后大鼠继续存活48 h。分别取材脊髓和背根神经节,冰冻切片,用四甲基联苯胺(TMB)和H2O2呈色。结果脊髓L1～S3节段背根神经节内(DRG)均出现染成阳性的神经细胞,以L6-S1节段DRG中阳线细胞为著;脊髓L6部至S1侧角(以L6多见)出现HRP阳性神经细胞。结论根据本次实验结果,明确了支配膀胱逼尿肌的低位中枢—骶副交感神经核(SPN)的位置,明确了膀胱传入神经的主要部分。Objective To locate bladder afferent and efferent elements within the spinal cord in rats by retrograde tracing. Methods WGA - HRP was injected into the bladder wall of normal male Sprague - Dawley rats （250 ~ 300 g）. 48 hours after the injection, the rats were killed, frozen sections were made from the spinal cord and dorsal root ganglian （DRG） and color developed with colorimetric tetramethyl benzidine （TMB） and I-I202. Results HRP positive neurons located in L1 - $3 DRG, mainly in 1..6 - S1 DRG. HRP - positive neurons were also found in the lateral horn of L6 - S1 （ more in 1.6）. Conclusion According to the experimental results, the lower nerve center - sacral parasympathetic nucleus （SPN） for the innervation of the detrusor of bladder was located and determined.

关 键 词：大鼠 脊髓损伤 神经源性膀胱 膀胱传入和传出神经 逆行示踪 

分 类 号：R651.2[医药卫生—外科学]