致病性马兽疫链球菌的分离鉴定、生物学特性及其M-like基因的序列分析  

Isolation and identification of a Streptococcus equi subsp. zooepidemicus and the M-like gene analysis

作　　者：王淑杰[1,2] 姜成钢[2] 武嘉男[2] 刘永刚[2] 金家民[1] 刘鹤[2] 蔡雪辉[2] 张秀英[1] 

机构地区：[1]东北农业大学动物医学院药理教研室,黑龙江哈尔滨150001 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/疫病诊断与技术服务中心,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2012年第11期878-881,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：转基因生物新品种培育重大专项(2009ZX08009-141B)

摘　　要：为鉴定锦州某猪场引起关节炎病疫情的病原,本研究将患猪关节液在血平板培养基上培养进行细菌分离;对分离细菌进行形态学观察、生化反应、致病性试验及药物敏感性试验;并对其M-like基因进行克隆和测序分析。结果在显微镜下观察到周围具有透明的β溶血环的革兰氏阳性中长链球菌,经生化反应及M-like基因鉴定为马兽疫链球菌(命名为10JZ12);分离株10JZ12对小鼠的致死剂量为1.75×108cfu;并对苯唑西林、青霉素G、红霉素、克拉霉素、克林霉素、米诺环素、环丙沙星、左氟沙星、多粘菌素B、呋喃妥因敏感,对氯霉素、四环素、诺氟沙星、复方新诺明不敏感。A pathogenic bacteria was isolated from pig synovia fluid of arthrosis by blood agar culture and characterized by morphological observation, biochemical test, M-like gene sequence analysis, antibiotic sensitive test and mice infection experiment. The morphological observation showed that the bacteria isolate was G^＋ Streptococcus and the M-like gene sequence analysis showed that the homology of the isolate was 96.2% to 97.1% with the Str.equi subsp, zooepidemicus reference strains, indicating that the bacteria isolate was S.equi subsp, zooepidemicus, designate 10JZ12. The lethal dose of the isolate was 1.75×10^8 cfu to mice, and the isolate was sensitive to oxacillin, penicillin G, erythrocin, clarithromycin, clindamycin, minocycline, ciprofloxacin, levofloxacin, polymyxin B and furadantin, but resistant to amphemycin, ambramycin, norfloxacin.

关 键 词：马兽疫链球菌 分离 鉴定 PCR 

分 类 号：S852.61[农业科学—基础兽医学]