唐古特大黄多糖组分1对电离辐射损伤小鼠造血系统的影响  

Protective effects of purified fraction 1 of polysaccharide extracted from rheum tanguticum on radiation-induced hematopoietic damage in mice

作　　者：刘琳娜[1] 郭志伟 张琰[1] 张甜[1] 张文娟[1] 戚志华[1] 徐媛[1] 

机构地区：[1]第四军医大学唐都医院药剂科 [2]新疆布尔津县人民医院

出　　处：《中国临床药理学与治疗学》2012年第10期1087-1090,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(81073135)

摘　　要：目的:探讨唐古特大黄多糖组分1(RTP1)对电离辐射损伤小鼠造血功能的影响。方法:采用雄性6周龄昆明种小鼠,随机分为5组:正常对照组(Normal Control,NC)、辐射对照组(Irradia-tion Control,IC)以及RTP1低剂量组(200mg/kg)、中剂量组(400mg/kg)和高剂量组(800mg/kg),采用灌胃给药方式,连续14d,NC组和IC组则给予等量的生理盐水,第14d除NC组外,各组小鼠均接受6.5Gy/只60Coγ射线照射1次,照射后继续灌胃给药,观察照射后30d小鼠生存情况,并对小鼠骨髓有核细胞数(bonemarrow cell,BMC)、骨髓DNA含量、内源性脾集落形成单位(spleen colony forming unit,CFU-S)及外周血中白细胞(white blood cell,WBC)计数进行检测。结果:与IC组比较,RTP1可提高小鼠30d存活率,并剂量依赖性地升高外周血中WBC计数、骨髓有核细胞数、DNA含量以及脾结节数。结论:RTP1对辐射小鼠的造血系统起到一定保护作用。AIM： To study the protective effects of purified fraction 1 of polysaccharide extracted from rheum tanguticum（RTP1） on radiation-induced hematopoietic damage in mice.METHODS： Male Kunming mice which were 6 week old were randomly divided into five groups： normal group（NC）,irradiation control group（IC）,RTP1 low dose group（200 mg/kg）,middle dose group（400 mg/kg） and high dose group（800 mg/kg）.The mice in RTP1 group were poured through gastic route by RTP1 for 14 d,the mice in NC and IC group were given by 0.9% sodium chloride solution through the same way.The mice in all groups except NC group were irradiated with 6.5 Gy 60 Co γ-ray on the fourteenth day.The 30 d survival rate of mice was observed.The number of white blood cell（WBC） and bone marrow cell（BMC）,as well as the count of endo-genous splenic colony-forming-unit（CFU-S） were detected.DNA content of marrow was also measured.RESULTS：Compared with that of the IC group,30 d survival rate,the number of WBC and BMC,the count of CFU-S and the DNA content of marrow were significantly higher with a dose-dependent manner.CONCLUSION： RTP1 could improve the hematopoietic recovery after irradiation.

关 键 词：唐古特大黄多糖 辐射损伤 60Coγ射线 造血系统 

分 类 号：R147[医药卫生—公共卫生与预防医学] R965.2