藏药夏果贝的生药鉴别及荧光显微镜的应用  

Normal light and fluorescence microscopy for authentication of Delphinii Brunoniani Herba of Tibet

作　　者：王亚琼[1] 徐福春[2] 卓玛东智[2] 刘鄂湖[1] 徐络珊[1] 刘惠娟[1] 李萍[1] 

机构地区：[1]中国药科大学,天然药物活性组分与药效国家重点实验室,江苏南京210009 [2]西藏大学医学院药学系,西藏拉萨850000

出　　处：《药学学报》2012年第11期1555-1560,共6页Acta Pharmaceutica Sinica

基　　金：天然药物活性组分与药效国家重点实验室开放课题资助项目(SKLNMKF201224);西藏大学青年科研培育基金项目(ZD11PZ01)

摘　　要：夏果贝(Delphinii Brunoniani Herba)来源于毛茛科翠雀属植物囊距翠雀花(Delphinium brunonianumRoyle)的干燥地上部分,为常用藏药之一。主治流感、皮肤痒疹、蛇咬伤等症。本文对藏药夏果贝的生药性状与显微特征进行了观察。通过对夏果贝的性状特征观察,同时利用普通光学显微镜和荧光显微镜对夏果贝茎、叶的横切面及粉末进行观察,寻找典型的显微特征。在夏果贝的叶、嫩茎及花萼下表面,发现了一种花柱状含黄色分泌物的单细胞腺毛。通过荧光显微镜对叶和茎的横切面观察,不需要经过复杂耗时的传统染色即可寻找到发出明显荧光的木质部和中柱鞘纤维群。研究结果为藏药夏果贝的生药鉴别提供了实验依据。Dried herb of Delphinium brunonianum Royle （Ranunculaceae） has long been used under the herbal name ＂Xiaguobei＂ （Delphinii Brunoniani Herba） in traditional Tibetan medicine and prescribed for the treatment of influenza, itchy skin rash and snake bites. In order to find a useful and convenient method for the identification of microscopic features, the technique of fluorescence microscopy was applied to authenticate ＂Xiaguobei＂ of Tibet. The transverse sections of stem and leaf, as well as the powder of ＂Xiaguobei＂ were observed to seek for typical microscopic features by normal light and fluorescence microscopy. A style-like, single-cell glandular hair containing yellow secretions on the leaf, young stem and sepal of ＂Xiaguobei＂ was found. Under the fluorescence microscope, the xylem and pericycle fiber group emitted significant fluorescence. This work indicated that fluorescence microscopy could be an useful additional method for the authentication work. Without the traditional dyeing methods, the main microscopic features could be easily found by fluorescence microscopy. The results provided reliable references for the authentication of＂Xiaguobei＂.

关 键 词：夏果贝 囊距翠雀花 生药鉴别 荧光显微法 

分 类 号：R931[医药卫生—生药学]