类风湿关节炎滑膜成纤维细胞对T淋巴细胞生物学行为的干预作用  

Effects of Rheumatoid Synovial Fibroblasts on Biological Properties of T Cells

作　　者：赵文君[1] 崔爽爽[1] 邢国胜[1] 于顺禄[1] 

机构地区：[1]天津市天津医院骨科研究所中心实验室,300211

出　　处：《天津医药》2012年第11期1092-1094,I0001,共4页Tianjin Medical Journal

基　　金：天津市应用基础研究计划项目(项目编号:07JCYBJ10500)

摘　　要：目的:探讨体外培养类风湿关节炎(RA)患者滑膜成纤维细胞对T淋巴细胞生物学行为的影响。方法:滑膜组织取自关节置换术的RA患者,酶消化法获得滑膜成纤维细胞并扩增培养,T淋巴细胞选用Jurkat T淋巴细胞株。将T淋巴细胞按培养方式分为单纯T淋巴细胞培养组及与RA滑膜成纤维细胞共育培养组2组。分别采用噻唑蓝(MTT)法、流式细胞仪和实时荧光定量PCR方法检测2组T淋巴细胞的扩增能力、凋亡率及白细胞介素(IL)-2 mRNA、IL-23p19 mRNA的表达水平。结果:共育培养组4d和7d时T淋巴细胞增殖率高于单纯培养组,而10d和13d时增殖率低于单纯培养组,差异均有统计学意义(P<0.01);共育培养组24h和48hT淋巴细胞凋亡率均低于单纯培养组,差异有统计学意义(P<0.05);共育培养组较单纯培养组T淋巴细胞表达较高的白细胞介素(IL)-2 mRNA(2.33±0.09vs1.00±0.12)和IL-23p19 mRNA(4.01±0.25vs1.00±0.15),差异均有统计学意义(t分别为15.03、17.78均P<0.01)。结论:RA滑膜成纤维细胞可诱导T淋巴细胞的激活,这可能是RA发病及进展的影响因素之一。Objective： To investigate the effect of rheumatoid synovial fthroblast on biological properties of T cells. Methods： The sample of synovial tissue was obtained from rheumatoid arthritis （RA） patients with joint replacement surgery. The synovial fibroblasts were extracted by enzyme. The Jurkat T cell line was used in the experiment. These T cells were divided into two groups, T cell alone group and co-culture group according to whether cells were co-cultured with RA fibroblast. MTT, flow cytometry and real-time PCR were used to detect the proliferation ability, the apoptosis and the expression of interleukin （IL）-2 mRNA and IL-23p19 mRNA of T cells, respectively. Results： The proliferation rate was significantly higher in co-cuhure group than that ofT cell alone group at the 4^th d and the 7^th d respectively （P 〈 0.01）, which was significantly lower in co-euhure group than that of T cell alone group at the 10^thd and the 13^thd respectively （P 〈 0.01）. The apoptotic rate was significantly lower in co-culture group than that of T cell alone group at the 24^thh and the 48^thh respectively （P 〈 0.05）. The expression levels of IL-2 mRNA （2.33±0.09 vs 1.00±0.12） and IL-23p19 mRNA （4.01±0.25 vs 1.00±0.15） were significantly higher in co-culture group than those in T cell alone group （P 〈 0.01）. Conclusion： RA fibroblasts have the potential effect on activating T cells, which may be one of factors involved in RA pathogenesis and progression.

关 键 词：关节炎 类风湿 滑膜 成纤维细胞 T淋巴细胞 淋巴细胞培养试验 混合 

分 类 号：R593.22[医药卫生—内科学]