白菜病毒诱导基因沉默技术体系的建立  

The Technology System Establishment of VIGS in Non-heading Chinese Cabbage

作　　者：杨学东[1] 戴薇[1] 张昌伟[1] 王金彦[1] 孔敏[1] 侯喜林[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,农业部华东地区园艺作物生物学与种质创制重点实验室,南京210095

出　　处：《园艺学报》2012年第11期2168-2174,共7页Acta Horticulturae Sinica

基　　金：江苏省自然科学基金项目(BK2009311);现代农业产业技术体系建设项目(国家大宗蔬菜产业技术体系CARS-25-A-12)

摘　　要：以白菜(Brassica campestrisssp.chinensis Makino)品种‘苏州青’为材料,利用芜菁黄化花叶病毒(Turnip yellow mosaic virus,TYMV)质粒pTY成功抑制了白菜叶片八氢番茄红素脱氢酶(phytoene desaturase,PDS)基因的表达,验证了病毒载体pTY在白菜上诱导基因沉默的能力,建立了白菜病毒诱导基因沉默VIGS体系。为了进一步验证病毒载体pTY在白菜上诱导基因沉默的能力,以BcNRT1为靶基因,构建pTY-BcNRT1质粒侵染白菜后,显著抑制了BcNRT1在转录水平的表达。In this study,a viral vector derived from Turnip yellow mosaic virus（TYMV）was used to induce virus-induced gene silencing（VIGS）of phytoene desaturase gene（PDS） in non-heading Chinese cabbage（Brassica campestris ssp. chinensis Makino.）cultivar‘Suzhouqing’and the result showed that the expression of PDS was successfully repressed. The ability of inducing silence was confirmed and the VIGS system was established. To further confirm the VIGS effect on non-heading Chinese cabbage,BcNRT1 was selected as the target gene,and the expression of BcNRT1 was successfully inhibited after inoculated.

关 键 词：白菜 芜菁黄化花叶病毒 病毒诱导基因沉默 

分 类 号：S634.3[农业科学—蔬菜学]