HBV Pre-S1蛋白与胎儿宫内感染  

HBV Pre-S1 protein and fetal intrauterine infection

作　　者：任新萍 胡娅莉[2] 薛金玲 

机构地区：[1]江苏省盐城市第一人民医院,224000 [2]南京大学附属鼓楼医院

出　　处：《中国妇幼保健》2012年第31期4906-4907,共2页Maternal and Child Health Care of China

摘　　要：目的:探讨HBV前S1蛋白与胎儿感染的相关性,为筛查HBsAg阳性孕产妇中可能感染胎儿的高危人群提供参考依据。方法:采用酶联免疫吸附试验(ELISA)双抗体夹心方法、分级定量PCR检测、酶联免疫吸附试验,检测256例HBV携带孕妇及其分娩的256例新生儿静脉血前S1蛋白、HBV DNA及HBV标志物。结果:①256例新生儿中发生HBV感染81例,感染率为31.6%。②前S1蛋白阳性孕产妇所分娩的85例新生儿中,感染率为88.2%(75/85),显著高于前S1蛋白阴性者的3.5%(6/171)(P<0.01)。③在感染的81例新生儿中,有79例其母前S1蛋白阳性(97.5%)(79/81),81例其母HBVDNA阳性,两者差异无统计学意义(P>0.01)。结论:①孕妇单纯HBsAg阳性,其婴儿感染率相对较低,如合并有前S1蛋白阳性或HBV DNA阳性,则胎盘传播率显著上升;②前S1蛋白检测可以代替血HBV DNA检测作为判断胎儿宫内感染的重要依据,可作为筛查HBsAg阳性孕产妇中易发生垂直传播人群的方法。Objective： To explore the correlation between HBV Pre - S1 protein and fetal infection, provide reference for screening the high risk population who may infect fetuses among the HBsAg positive pregnant women. Methods： ELISA double antibody sandwich method, grading quantitative PCR, and ELISA were used to detect HBV Pre - S1 protein, HBV DNA, and HBV markers in venous blood samples of 256 pregnant women carrying HBV and 256 neonates born by them. Results： Among 256 neonates, 81 neonates were infected by HBV, the infection rate was 31.6%. Among 85 neonates born by pregnant women with positive HBV Pre - S1 protein, 75 neonates were infected, the infection rate was 88.2%, which was statistically significantly higher than that in neonates born by pregnant women with negative HBV Pre- S1 protein [ 3.5 % （6/171）] （P 〈 0. 01 ） . Among 81 infected neonates, the mothers of 71 neonates were found with positive HBV Pre- S1 protein [97.5% （79/81）], and the mothers of 81 neonates were found with positive HBV DNA, there was no statistically significant difference （P 〉0. O1 ） . Conclusion： The infection rate of HBV in neonates whose mothers are found with simple positive HBsAg is low, if the mothers are found with positive HBsAg combined with positive HBV Pre - S1 protein or positive HBV DNA, the transplacental transmission rate of HBV increases significantly ; as an important criterion to predict fetal intrauterine infection, HBV Pre - S1 protein detection can replace HBV DNA detection, which can be used as a method to screen the population who are easy to occur vertical transmission among pregnant women with positive HBsAg.

关 键 词：乙型肝炎病毒 前S1蛋白 胎儿感染 

分 类 号：R714[医药卫生—妇产科学]