K_(ATP)开放剂对新生鼠缺氧缺血性脑损伤的保护作用及其机制研究  

Study on the protective effect of K_(ATP) opener on neonatal rats with hypoxic-ischemic brain damage and the mechanism

作　　者：黄广[1] 王銮虹[2] 

机构地区：[1]汕头大学医学院第一附属医院儿科,广东汕头515041 [2]汕头大学医学院附属肿瘤医院妇瘤科

出　　处：《中国妇幼保健》2012年第31期4962-4964,共3页Maternal and Child Health Care of China

基　　金：广东省重点科技攻关项目〔2KM05604S〕

摘　　要：目的:探讨KATP开放剂对新生大鼠缺氧缺血性脑损伤的保护作用及其作用机制。方法:制作新生大鼠缺氧缺血性脑损伤(HIBD)模型,制模后予KATP开放剂克罗卡林进行干预处理,检测大鼠体重的改变,死亡率以及脑组织中谷氨酸(Glu)及天门冬氨酸(Asp)含量。结果:KATP开放剂克罗卡林能增加HIBD大鼠体重增长值,减轻死亡率,减少HIBD脑组织中Glu及Asp的含量(P<0.05);KATP阻断剂格列本脲明显拮抗克罗卡林的上述作用。结论:KATP开放剂克罗卡林对HIBD具有一定的保护作用,其作用机制可能与激活ATP敏感性钾通道,减少兴奋性氨基酸的释放有关。Objective： To explore the protective effect of K_ATP opener on neonatal rats with hypoxic - ischemic brain damage （HIBD） and the mechanism. Methods： HIDB models of neonatal rats were established, then K_ATP opener Cromakalim was used for intervention and treatment, the change of body weight, mortality, the contents of Glu and Asp in the neonatal rats were detected. Results： K_ATP opener Cromakalim increased body weight of HIBD rats, reduced the mortality and the contents of Glu and Asp in cerebral tissue of HIBD rats （P 〈 0.05 ） . K_ATP blocker Glibenclamide antagonized the above - mentioned effects of Cromakalim. Conclusion： K_ATP opener Cromakalim has a certain protective effect on HIBD rats, and the mechanism may be correlated with activation of ATP sensitive potassium channel and reduce of the release of excitatory amino acids.

关 键 词：克罗卡林 脑缺氧 脑缺血 保护 兴奋性氨基酸 

分 类 号：R332[医药卫生—人体生理学]